Abstract: Conducted with light microscope,the epiderdermal and dermal features as well as the stomata index were investigated in 3 species in Pugionium Gaertn.—P.cornutum (L.) Gaertn.,P.calcaratum Kom. and P.dolatum Maxim. var. dolabratum,and the significant tests for data were carried out. The showed that in Pugionium Gaertn.,the epidermal cells are anomo-shaped, and stomata distribute in both the upper epidermis and the lower epidermis ,which are anisocytic type. The guard cells are kidney shaped and the inner cell walls are thickened to different degrees: P.dolatum Maxim. var. dolabratum＞P.calcaratum Kom.＞P.cornutum (L.) Gaertn.. The stomata are spindle shaped and sink as well.The stomata make and break showed a two-kurtosis carve in 3 species in a day. The stomata characters are very similar in P.calcaratum Kom. and P.dolatum Maxim. var. dolabratum,but shows a significant difference in P.cornutum (L.) Gaertn.,which indicate that microcosmic characters such as the stable stomata characters and its morphology could be important features in determing and evaluating genetic resources.

Key words: Pugionium Gaertn., stoma, leaf