Abstract: The study uses the gametocidal chromosome 2S derived from Ae.speltoides, inducing chromosonie breaks in Triticum aestivum ningnong and 5R/5A wheat-rye substitution lines.Observation of the meiosis of the hybrid F₁,in metaphaseⅠand Ⅱ,there are appearing the high-frequency of univalents and multivalents;in anaphaseⅠand Ⅱ,there are forming many chromosomal abnormalities such as a large number of lagging chromosomes,chromosomal fragments and chromosomal bridges;in dispores and tetraspores,there exist micronucleus.In this experiment, the meiosis behavior of GCl substitution lines hybrid with 5R/5A subtitution lines is more complex than of ningnong F₁ hybrid. Through statistical analysis, the dual function on meiosis behavior,which is the hybrid between the gametocidal substitution lines and the 5R/5A substitution lines,is more markable than the sole gametocidal substitution lines function.Therefor，the use of the dual function on inducing chromosome translocation is an effective way.

Key words: gametocidal chromosome, substitution lines, meiosis