Abstract: Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis. Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg·L^-1 benzyladenine (BA) with or without 1~10 mg·L^-1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction frequency of adventitious buds (90%) was obtained on DCR medium containing 1 mg·L^-1 BA. Subsequent transfer of buds to plant growth regulator (PGR)-free medium in time was advantageous for elongation of adventitious buds. 1/2DCR medium with 1 mg·L^-1 indole-3-butyric acid (IBA) was suitable for adventitious root initiation, on which average rooting frequency was 47.1%. Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse, where they had a survival rate of 86.4% and no morphological abnormalities.

Key words: Chinese pine, micropropagation, adventitious bud, rooting, acclimatization