%0 Journal Article
%A KONG Dong-Mei
%A WAN Ting
%T Direct Organogenesis and Plantlet Regeneration from Mature Zygotic Embryos of Chinese Pine(<em>Pinus tabulaeformis</em> Carr.)
%D 2010
%R 10.7525/j.issn.1673-5102.2010.06.008
%J Bulletin of Botanical Research
%P 668-673
%V 30
%N 6
%X Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis. Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg&middot;L<sup>-1</sup> benzyladenine (BA) with or without 1~10 mg&middot;L<sup>-1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction frequency of adventitious buds (90%) was obtained on DCR medium containing 1 mg&middot;L<sup>-1</sup> BA. Subsequent transfer of buds to plant growth regulator (PGR)-free medium in time was advantageous for elongation of adventitious buds. 1/2DCR medium with 1 mg&middot;L<sup>-1</sup> indole-3-butyric acid (IBA) was suitable for adventitious root initiation, on which average rooting frequency was 47.1%. Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse, where they had a survival rate of 86.4% and no morphological abnormalities.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2010.06.008