Construction of prokaryotic expression vector of Arabidopsis pumila chitinase and its expression in Escherichia coil

doi:10.7525/j.issn.1673-5102.2005.04.009

Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 419-422.doi: 10.7525/j.issn.1673-5102.2005.04.009

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Construction of prokaryotic expression vector of Arabidopsis pumila chitinase and its expression in Escherichia coil

ZHU Xin-Xia¹, TIAN Li-Ping², GAO Jian-Feng², ZHU Jian-Bo¹

1. Key Laboratory of Oasis Ecology Agriculture of Xinjiang Bingtuan, Shihezi 832003;
2. College of Bio-engineering, Shihezi University, Shihezi 832003

Received:2004-09-17 Online:2005-12-15 Published:2016-06-14

Abstract

Abstract: Prokaryotic expression vector pET-CH of Arabidopsis pumila chitinase gene cDNA was constructed by means of recombinant gene technology and expressed in Escherichia coil BL21 under induction of IPTG, the expressed products were detected by SDS-PAGE analysis.Result show: Recombinant chitinase was efficiently expressed in E. coli BL21, it molecular weight was about 40 KD.The successful construction of prokaryotic expression vector containing Arabidopsis pumila chitinase gene and effective expression of recombinant chitinase in E. coli BL21 laid the foundation for further study on its biological function.

Key words: Arabidopsis pumila, chitinase, cDNA cloning vectors, gene expression

ZHU Xin-Xia, TIAN Li-Ping, GAO Jian-Feng, ZHU Jian-Bo. Construction of prokaryotic expression vector of Arabidopsis pumila chitinase and its expression in Escherichia coil[J]. Bulletin of Botanical Research, 2005, 25(4): 419-422.

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Construction of prokaryotic expression vector of Arabidopsis pumila chitinase and its expression in Escherichia coil

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