植物研究 ›› 2021, Vol. 41 ›› Issue (6): 928-937.doi: 10.7525/j.issn.1673-5102.2021.06.011
收稿日期:2020-04-08
									
				
									
				
									
				
											出版日期:2021-11-20
									
				
											发布日期:2021-10-29
									
			通讯作者:
					张芹
											E-mail:zqin166@163.com
												作者简介:刘金玉(1994—),女,硕士研究生,主要从事园林植物栽培及逆境生理研究。
				
							基金资助:
        
               		Jin-Yu LIU1, Sheng-Wei LIN1, Jing-Yu PAN2, Qin ZHANG1(
)
			  
			
			
			
                
        
    
Received:2020-04-08
									
				
									
				
									
				
											Online:2021-11-20
									
				
											Published:2021-10-29
									
			Contact:
					Qin ZHANG   
											E-mail:zqin166@163.com
												About author:LIU Jin-Yu(1994—),female,master,mainly engaged in landscape plants cultivation and adversity physiology.				
							Supported by:摘要:
为提高金莲花育苗的幼苗质量,筛选适宜推广应用的金莲花育苗基质配方,探索栽培基质对金莲花幼苗生长影响的生理机制,本试验以金莲花幼苗为植物材料进行盆栽试验,研究了不同比例的草炭、蛭石和珍珠岩改良园土对金莲花幼苗生长、生理和光合特性的影响。结果表明:添加草炭后T4、T5、T7对园土的理化性质有明显改善,其中以处理T7(草炭∶珍珠岩∶蛭石∶园土=1∶1∶1∶1)改善效果最好,金莲花幼苗的株高、冠幅、叶面积、叶片数量分别比对照提高32.08%、26.62%、56.22%、64.41%;显著提高了金莲花叶片的抗氧化酶活性,矿质营养含量,叶绿素含量及光合速率(P<0.05),可作为金莲花育苗的基质配方。
中图分类号:
刘金玉, 林声威, 潘景玉, 张芹. 不同土壤改良基质对金莲花幼苗生长及生理指标的影响[J]. 植物研究, 2021, 41(6): 928-937.
Jin-Yu LIU, Sheng-Wei LIN, Jing-Yu PAN, Qin ZHANG. Different Improved Soil Substrates on the Growth and Physiological Characters of Seedlingsfrom Trollius chinensis Bunge[J]. Bulletin of Botanical Research, 2021, 41(6): 928-937.
表1
土壤改良方式
处理编号 Treatment  | 土壤基质配比 Soil substrate ratio  | 
|---|---|
| CK | 园土Garden soil | 
| T1 | 园土+草炭Garden soil+peat(1∶1) | 
| T2 | 园土+蛭石Garden soil+vermiculite(1∶1) | 
| T3 | 园土+珍珠岩Garden soil+perlited(1∶1) | 
| T4 | 园土+草炭+蛭石 Garden soil+peat+vermiculite(1∶1∶1)  | 
| T5 | 园土+草炭+珍珠岩 Garden soil+peat+perlited(1∶1∶1)  | 
| T6 | 园土+蛭石+珍珠岩 Garden soil+vermiculite+perlited(1∶1∶1)  | 
| T7 | 园土+草炭+蛭石+珍珠岩 Garden soil+peat+vermiculite+perlited(1∶1∶1∶1)  | 
表2
不同土壤改良基质的物理性质
处理 Treatment  | 容重 Bulk density(g·cm-3)  | 总孔隙度 Total porosity(%)  | 通气孔隙 Aeration porosity(%)  | 大小孔隙比 Porosity ratio  | 
|---|---|---|---|---|
| CK | 0.95±0.008a | 65.70±0.300bc | 13.36±2.360f | 0.26±0.058f | 
| T1 | 0.63±0.014bc | 67.24±0.240bc | 24.74±0.740cd | 0.58±0.024bcd | 
| T2 | 0.72±0.002b | 64.68±0.325bc | 22.40±0.400de | 0.53±0.019cde | 
| T3 | 0.62±0.015bc | 62.83±0.175c | 17.78±0.220ef | 0.39±0.005ef | 
| T4 | 0.73±0.044b | 69.32±2.320b | 25.78±0.780cd | 0.59±0.003bcd | 
| T5 | 0.46±0.020d | 67.76±0.760bc | 29.19±0.190ab | 0.76±0.006a | 
| T6 | 0.53±0.015cd | 65.35±0.350bc | 20.60±0.400de | 0.46±0.017de | 
| T7 | 0.43±0.029d | 75.72±0.720a | 31.40±1.400ab | 0.71±0.043ab | 
表3
不同土壤改良基质的化学性质
处理 Treatment  | 碱解氮 Available N(mg·kg-1)  | 速效磷 Available P(mg·kg-1)  | 速效钾 Available K(mg·kg-1)  | pH值 Potential of hydrogen  | EC值 Electrical conductivity(μs·cm-1)  | 
|---|---|---|---|---|---|
| CK | 75.03±1.512c | 33.61±2.055d | 82.16±0.605d | 7.06±0.055a | 208.98±0.975e | 
| T1 | 118.87±1.674ab | 50.76±1.755bc | 247.61±5.830a | 6.69±0.190a | 333.65±1.650b | 
| T2 | 53.15±1.415de | 37.51±2.323d | 160.99±5.379b | 7.05±0.050a | 187.01±1.010fg | 
| T3 | 45.80±2.697e | 37.37±1.284d | 110.07±6.522cd | 6.57±0.065a | 183.23±1.225g | 
| T4 | 97.71±0.924b | 52.61±0.843bc | 252.23±1.110a | 6.59±0.010a | 322.58±0.425c | 
| T5 | 111.57±5.544ab | 64.31±2.006a | 264.66±6.820a | 7.08±0.080a | 307.69±0.690d | 
| T6 | 66.23±1.003cd | 44.30±2.886cd | 135.75±2.145bc | 6.88±0.075a | 191.99±1.985f | 
| T7 | 110.30±5.190ab | 58.79±1.990ab | 254.54±5.280a | 6.88±0.025a | 481.27±1.265a | 
表4
不同土壤改良基质对金莲花幼苗叶绿素含量的影响
处理 Treatment  | 叶绿素a含量 Chlorophyll a (mg·g-1)  | 叶绿素b含量 Chlorophyll b (mg·g-1)  | 叶绿素总量 Total chlorophyll (mg·g-1)  | 
|---|---|---|---|
| CK | 0.80±0.582d | 0.24±0.575a | 1.14±0.870e | 
| T1 | 1.17±0.576abc | 0.37±0.417a | 1.63±1.141b | 
| T2 | 1.19±0.424abc | 0.37±0.878a | 1.63±1.745b | 
| T3 | 0.98±1.044cd | 0.31±0.730a | 1.35±0.546d | 
| T4 | 1.27±0.625ab | 0.32±0.212a | 1.63±1.148b | 
| T5 | 1.05±0.233bcd | 0.37±0.145a | 1.47±1.530c | 
| T6 | 0.86±0.442d | 0.28±0.350a | 1.17±1.062e | 
| T7 | 1.40±0.452a | 0.42±0.688a | 1.89±0.353a | 
表5
不同土壤改良基质下各指标相关系数
相关系数 Correlation coefficient  | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | 
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X1 | 1 | ||||||||||||
| X2 | -0.45 | 1 | |||||||||||
| X3 | -0.76* | 0.76* | 1 | ||||||||||
| X4 | -0.76* | 0.65 | 0.98** | 1 | |||||||||
| X5 | -0.36 | 0.72* | 0.71* | 0.69 | 1 | ||||||||
| X6 | -0.73* | 0.70 | 0.92** | 0.93** | 0.83* | 1 | |||||||
| X7 | -0.57 | 0.69 | 0.94** | 0.93** | 0.85** | 0.92** | 1 | ||||||
| X8 | 0.06 | 0.03 | -0.01 | 0.06 | 0.002 | 0.01 | -0.13 | 1 | |||||
| X9 | -0.50 | 0.96** | 0.81* | 0.72* | 0.83* | 0.78* | 0.80* | -0.11 | 1 | ||||
| X10 | -0.76* | 0.78* | 0.72* | 0.67 | 0.57 | 0.74* | 0.53 | 0.32 | 0.71* | 1 | |||
| X11 | -0.21 | 0.76* | 0.26 | 0.11 | 0.35 | 0.28 | 0.17 | -0.20 | 0.70 | 0.55 | 1 | ||
| X12 | -0.52 | 0.91** | 0.65 | 0.52 | 0.51 | 0.62 | 0.52 | -0.07 | 0.81* | 0.81* | 0.80* | 1 | |
| X13 | -0.53 | 0.93** | 0.72* | 0.61 | 0.61 | 0.70 | 0.62 | -0.17 | 0.92** | 0.74* | 0.85** | 0.91** | 1 | 
| X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | |
| X14 | -0.61 | 0.69 | 0.89** | 0.85** | 0.45 | 0.70 | 0.76* | 0.14 | 0.63 | 0.65 | 0.14 | 0.65 | 0.57 | 
| X15 | -0.54 | 0.89** | 0.88** | 0.82* | 0.67 | 0.84** | 0.80* | 0.11 | 0.83* | 0.77* | 0.48 | 0.85** | 0.82* | 
| X16 | -0.54 | -0.80* | -0.88** | -0.84** | -0.51 | -0.74* | -0.75* | -0.22 | -0.73* | -0.69 | -0.33 | -0.72* | -0.72* | 
| X17 | -0.63 | 0.71 | 0.51 | 0.39 | 0.22 | 0.34 | 0.25 | 0.12 | 0.61 | 0.80* | 0.66 | 0.77* | 0.68 | 
| X18 | -0.56 | 0.88** | 0.84** | 0.79* | 0.83* | 0.89** | 0.82* | 0.14 | 0.85** | 0.84** | 0.47 | 0.83* | 0.78* | 
| X19 | -0.59 | 0.93** | 0.82* | 0.75* | 0.79* | 0.86** | 0.78* | 0.01 | 0.89** | 0.85** | 0.62 | 0.91** | 0.87** | 
| X20 | -0.69 | 0.91** | 0.92** | 0.87** | 0.82* | 0.92** | 0.85** | 0.09 | 0.92** | 0.88** | 0.52 | 0.83* | 0.85** | 
| X21 | -0.42 | 0.72* | 0.81* | 0.74* | 0.52 | 0.57 | 0.77* | -0.18 | 0.78* | 0.38 | 0.32 | 0.50 | 0.65 | 
| X22 | -0.49 | 0.91** | 0.85** | 0.76* | 0.59 | 0.71* | 0.75* | 0.07 | 0.83* | 0.72* | 0.50 | 0.86** | 0.81* | 
| X14 | X15 | X16 | X17 | X18 | X19 | X20 | X21 | X22 | |||||
| X14 | 1 | ||||||||||||
| X15 | 0.87** | 1 | |||||||||||
| X16 | -0.93** | -0.94** | 1 | ||||||||||
| X17 | 0.55 | 0.53 | -0.54 | 1 | |||||||||
| X18 | 0.77* | 0.94** | -0.81* | 0.52 | 1 | ||||||||
| X19 | 0.73* | 0.94** | -0.79* | 0.61 | 0.98** | 1 | |||||||
| X20 | 0.79* | 0.93** | -0.84** | 0.63 | 0.96** | 0.97** | 1 | ||||||
| X21 | 0.75* | 0.67 | -0.75* | 0.48 | 0.55 | 0.57 | 0.68 | 1 | |||||
| X22 | 0.91** | 0.96** | -0.95** | 0.64 | 0.88** | 0.89** | 0.88** | 0.77* | 1 | 
| 1 | 李良千.毛茛科金莲花亚科植物的地理分布[J].植物分类学报,1995,33(6):537-555. | 
| Li L Q.The geographical distribution of Subfam.Helleboroideae(Ranunculaceae)[J].Acta Phytotaxonomica Sinica,1995,33(6):537-555. | |
| 2 | 丁万隆,陈震,陈君.金莲花属药用植物资源及利用[J].中国野生植物资源,2003,22(6):19-21. | 
| Ding W L,Chen Z,Chen J.Resources and utilization of medicinal plants of Trollius L.[J].Chinese Wild plant Resources,2003,22(6):19-21. | |
| 3 | 连兆煌.无土栽培技术与原理[M].北京:中国农业出版社,1994. | 
| Lian Z H.Soilless cultivation techniques and principles[M].Beijing:China Agriculture Press,1994. | |
| 4 | 中国土壤学会农业化学专业委员会.土壤农业化学常规分析方法[M].北京:科学出版社,1983. | 
| Agricultural Chemical Specialized Committee Chinese Soil Society.Methods for routine analysis of soil agrochemistry[M].Beijing:Science Press,1983. | |
| 5 | 李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2000. | 
| Li H S.Principle and technology of plant physiology and biochemistry experiment[M].Beijing:Higher Education Press,2000. | |
| 6 | 张志良.植物生理学实验指导:4版[M].北京:高等教育出版社,2009. | 
| Zhang Z L.Experimental guidance of plant physiology:4th ed[M].Beijing:Higher Education Press,2009. | |
| 7 | 康红梅,张启翔,唐菁.栽培基质的研究进展[J].土壤通报,2005,36(1):124-127. | 
| Kang H M,Zhang Q X,Tang J.Research advances on growth media[J].Chinese Journal of Soil Science,2005,36(1):124-127. | |
| 8 | 张芹.仙客来盆花温室规模化生产栽培基质筛选的研究[D].北京:北京林业大学,2004. | 
| Zhang Q.Studies on soilless substrates used in pot cyclamen production[D].Beijing:Beijing Forestry University,2004. | |
| 9 | 何宛晟,卢广林,赵权,等.不同改良剂对人参土壤及其生长发育的影响[J].分子植物育种,2018,16(20):6826-6831. | 
| He W C,Lu G L,Zhao Q,et al.Effects of different modifiers on ginseng soil and its growth and development[J].Molecular Plant Breeding,2018,16(20):6826-6831. | |
| 10 | 刘芳,袁雅丽.不同土壤改良方式对乌柳生长的影响[J].防护林科技,2016,(8):4-7. | 
| Liu F,Yuan Y L.Effects of different soil amelioration on growth of Salix cheiophila[J].Protection Forest Science and Technology,2016,(8):4-7. | |
| 11 | 张鋆鋆,刘冰洋,王一凡,等.植物根系研究进展[J].天津农业科学,2016,22(11):11-18. | 
| Zhang Y Y,Liu B Y,Wang Y F,et al.Research progress of plant roots[J].Tianjin Agricultural Sciences,2016,22(11):11-18. | |
| 12 | Fridovich I.The biology of oxygen radicals[J].Science,1978,201(4359):875-880. | 
| 13 | 缪淼.外源物质和土壤改良对盐土上马蔺生长及生理特性的影响[D].南京:南京农业大学,2017. | 
| Miao M.Effects of exogenous substance and soil improvement on growth and physiological characteristics of Iris lactea var. chinensis in salinity soil[D].Nanjing:Nanjing Agricultural University,2017. | |
| 14 | 田侠,郑庆柱,高雪,等.土壤改良剂对干旱胁迫下青稞幼苗形态及生理特性的影响[J].山东农业科学,2019,51(2):67-71. | 
| Tian X,Zheng Q Z,Gao X,et al.Effect of soil conditioners on morphological and physiological characteristics of highland barley seedlings under drought stress[J].Shandong Agricultural Sciences,2019,51(2):67-71. | |
| 15 | 陈少裕,刘杰.水分胁迫对甘蔗叶片线粒体膜流动性的影响及其与膜脂过氧化的关系[J].植物生理学报,1991,17(3):285-289. | 
| Chen S Y,Liu J.The effect of water stress on membrane fluidity of leaf mitochondria of sugarcane and its relation to membrane lipid peroxidation[J].Acta Phytophysiologica Sinica,1991,17(3):285-289. | |
| 16 | Reddy A R,Chaitanya K V,Vivekanandan M.Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants[J].Journal of Plant Physiology,2004,161(11):1189-1202. | 
| 17 | 杨婷.膜脂过氧化对植物细胞的伤害[J].科技与创新,2018,(8):61-62. | 
| Yang T.Damage of membrane lipid peroxidation to plant cells[J].Science and Technology & Innovation,2018,(8):61-62. | |
| 18 | 程朝霞,昝亚玲,张秀红.不同配比的有机肥和河沙对盐碱土改良效果及对观赏向日葵生长的影响[J].分子植物育种,2019,17(17):5867-5873. | 
| Cheng Z X,Zan Y L,Zhang X H.Improvement effects of organic fertilizer and river sand with different proportion to saline-alkali soil and ornamental sunflower[J].Molecular Plant Breeding,2019,17(17):5867-5873. | |
| 19 | 唐雪东,李亚东,吴林,等.土壤改良对越桔(Vaccimium)某些生理指标的影响[J].沈阳农业大学学报,2003,34(6):419-422. | 
| Tang X D,Li Y D,Wu L,et al.Effect of soil on physiological characters of blueberry-Vaccimium[J].Journal of Shenyang Agricultural University,2003,34(6):419-422. | |
| 20 | 张运红,和爱玲,杨占平,等.土壤改良剂对镉污染土壤小麦抗性、光合特性及产量的影响[J].河南农业科学,2018,47(12):57-63. | 
| Zhang Y H,He A L,Yang Z P,et al.Effects of soil amendments on resistance,photosynthetic characteristics and yield of wheat in cadmium-contaminated soils[J].Journal of Henan Agricultural Sciences,2018,47(12):57-63. | |
| 21 | 乔维范,袁雅丽.高寒冻土层地区不同土壤改良措施对花叶海棠光合特性的影响[J].防护林科技,2017,(5):66-69. | 
| Qiao W F,Yuan Y L.Effects of different soil improvement measures on photosynthetic characteristics of Malus transitoria in alpine permafrost[J].Protection Forest Science and Technology,2017,(5):66-69. | 
| [1] | 陈家兴, 王姝, 陈林丽, 侯夏丽, 杨庆祝, 尹任娅. 干旱条件对鬼针草和醉鱼草种间相互作用及生长的影响[J]. 植物研究, 2023, 43(5): 720-728. | 
| [2] | 郑晟, 高海霞, 苏敏, 卢尚欢, 张腾国, 武国凡. 外源蔗糖影响AtKEA1和AtKEA2调节拟南芥幼苗根的生长[J]. 植物研究, 2023, 43(4): 562-571. | 
| [3] | 曹俐, 杨蕴力, 李天芳, 姜静. 转BpGLK裂叶桦叶色及生长变异分析[J]. 植物研究, 2023, 43(3): 351-360. | 
| [4] | 唐莹莹, 郭传超, 石荡, 蒋南林, 许正, 刘立强. 果肉和埋土深度对新疆野杏种子萌发与幼苗生长的影响[J]. 植物研究, 2023, 43(2): 251-260. | 
| [5] | 令狐克念, 王姝. 不同生长阶段苘麻生物量分配对种群密度和土壤水分的响应[J]. 植物研究, 2023, 43(2): 272-280. | 
| [6] | 孙军, 李贵生. 粗梗水蕨营养期和生殖期微RNA的测定与分析[J]. 植物研究, 2022, 42(6): 1014-1022. | 
| [7] | 吴静, 王媛媛, 王丹妮, 刘百超, 刘中原. 刚毛柽柳ThGRF2基因的克隆和渗透胁迫应答分析[J]. 植物研究, 2022, 42(6): 1044-1051. | 
| [8] | 刘英, 吴嘉仪, 金玲, 季倩如, 付玉杰, 李德文. 遮光胁迫下外源NO对盆栽长春花土壤养分含量及幼苗生长特征的影响[J]. 植物研究, 2022, 42(6): 1088-1095. | 
| [9] | 曹小路, 赵巧竹, 幸华, 栗孟飞. 桃儿七种子解剖结构及其萌发生长期形态特征[J]. 植物研究, 2022, 42(5): 746-752. | 
| [10] | 王欢, 徐云飞, 刘一伯, 刘沁松, 徐文娟, 龙芸, 胥晓. 珙桐—灯台树枝和叶的水提物对白菜种子萌发和幼苗生长的化感效应[J]. 植物研究, 2022, 42(5): 866-875. | 
| [11] | 庄伟伟, 王明明. 荒漠地区8种草本植物营养元素含量的比较分析[J]. 植物研究, 2022, 42(5): 896-909. | 
| [12] | 龚莉, 翟伟, 吕丹, 张世航, 戈玉莹, 洪志, 陶冶. 不同生境入侵植物北美车前繁殖器官性状变异与权衡特征[J]. 植物研究, 2022, 42(4): 544-555. | 
| [13] | 高冰, 高鹏飞, 范卫芳, 冯郑红, 吴建慧. 镉胁迫对绢毛委陵菜-丛枝菌根真菌共生体根系结构和生理的影响[J]. 植物研究, 2022, 42(4): 647-656. | 
| [14] | 杨会肖, 徐放, 杨晓慧, 廖焕琴, 张卫华, 潘文. 不同水肥处理对尾叶桉苗木生长及生物量分配的影响[J]. 植物研究, 2022, 42(4): 667-676. | 
| [15] | 白舒冰, 邢小艺, 关雯雨, 董丽. 首云铁矿废弃土壤对2种景天属植物生长的影响[J]. 植物研究, 2022, 42(4): 677-687. | 
| 阅读次数 | ||||||
| 
												        	全文 | 
											        	
												        	 | 
													|||||
| 
												        	摘要 | 
												        
															 | 
													|||||