寄生植物锁阳种子萌发方法及愈伤组织、初生吸器诱导研究

doi:10.7525/j.issn.1673-5102.2020.06.007

植物研究 ›› 2020, Vol. 40 ›› Issue (6): 846-854.doi: 10.7525/j.issn.1673-5102.2020.06.007

寄生植物锁阳种子萌发方法及愈伤组织、初生吸器诱导研究

岳鑫,陈贵林

^1.内蒙古大学生命科学学院，呼和浩特 010021
^2.内蒙古医科大学药学院，呼和浩特 010021

收稿日期:2019-12-20 出版日期:2020-11-20 发布日期:2020-11-04
作者简介:岳鑫（1983—），女，讲师，理学博士，主要从事中药生物技术研究。
基金资助:
内蒙古自治区自然科学基金博士基金项目(2016BS0811);内蒙古自治区科技创新引导奖励资金项目(2015cztxyd02);教育部高等学校博士点基金(20091501110002);内蒙古科技成果转化项目(CGZH2018127)

In vitro Germination,Callus Induction and Primary Haustorium Organogenesis in the Parasitic Plant Cynomorium songaricum

Xin YUE¹^,², Gui-Lin CHEN¹()

^1.School of Life Science，Inner Mongolia University，Hohhot 010021
^2.College of Pharmacy，Inner Mongolia Medical University，Hohhot 010021

Received:2019-12-20 Online:2020-11-20 Published:2020-11-04
Contact: Gui-Lin CHEN E-mail:guilinchen61@163.com
About author:YUE Xin(1983—),female,lecturer,Ph.D.,mainly engaged in biotechnology research on Traditional Chinese Medicine.
Supported by:
Inner Mongolia Natural Science Foundation, PhD Foundation Project(2016BS0811);Inner Mongolia Science and Technology Innovation Guided Award Fund Project(2015cztxyd02);Specialized Research Fund for Doctoral Programs in Colleges and Universities(20091501110002);Inner Mongolia Science and Technology Achievements Transformation Project(CGZH2018127)

摘要/Abstract

摘要：

专性寄生植物锁阳以干燥肉质茎入药，为常用中药材。在锁阳的生活史中，需要经历种子萌发、芽管状器管伸长、初生吸器形成、次生吸器形成等过程，其中种子萌发和初生吸器形成是锁阳完成生活史的最基本条件。目前，触发锁阳种子萌发、初生吸器形成的植物生长调节物质的阈值和种类并不清楚，导致人工调控锁阳生活史困难及栽培收益不高。本论文运用组织培养方法，研究赤霉素、生长素和细胞分类素等多种激素交互作用对锁阳种子萌发和吸器形成的影响。研究结果显示：（1）多种激素的共同作用促进锁阳球形原胚的发育。（2）B₅培养基添加1.0 mg·L^-1 2，4-D，0.5 mg·L^-1 KT，1.0 mg·L^-1 GA₃可以高效诱导锁阳种子愈伤组织形成，愈伤诱导率达13.7％±3.1%。（3）B₅培养基添加0.5 mg·L^-1 2，4-D，0.25 mg·L^-1 KT可以高效诱导锁阳愈伤组织分化初生吸器，一些初生吸器继续分化成芽管状气管，延伸3~4 cm后，顶端膨大，形成新的初生吸器。本论文研究结果可为进一步研究锁阳种子萌发、初生吸器形成的内源激素变化规律及发生机制研究奠定基础。

关键词: 药用植物, 吸器诱导因子, 组织学, 寄主—寄生植物互作, 栽培

Abstract:

Cynomorium songaricum Rupr. is an obligate parasitic plant widely used as traditional Chinese medicine and Mongolian medicine. Here， we firstly describe protocols for in vitro germination， callus induction and haustorium organogenesis in C.songaricum Rupr. In this study， adequate concentrations of gibberellic acid（GA₃），_{in combination with other plant growth regulators in the medium， promoted embryo development and germination of C.songaricum seeds. The highest callus induction rate from seeds（13.7%） after a 40 d incubation was obtained with B₅ medium adding 2，4-dichlorophenoxyacetic acid（2，4-D； 1.0 mg·L^-1）， kinetin（KT；0.5 mg·L^-1） and GA₃（1.0 mg·L^-1. This resulted in callus_{formation in 13.7%±3.1% of seeds. Addition of 2，4-D（0.5 mg·L^-1） and KT（0.25 mg·L^-1） yielded highest haustorium organogenesis from calluses. Some primary haustorium branched to form adventitious roots of 3-4 cm in length. Subsequently， the tip of each adventitious root formed a nascent primary haustorium， which was then branched out into adventitious roots. The role of auxins（2，4-D in this study） in the formation of primary haustorium and adventitious roots from seed callus in C.songaricum was also discussed.}}

Key words: medicinal plant, HIFs, histology, host-parasite relationship, cultivation

中图分类号:

Q949.762.9

岳鑫, 陈贵林. 寄生植物锁阳种子萌发方法及愈伤组织、初生吸器诱导研究[J]. 植物研究, 2020, 40(6): 846-854.

Xin YUE, Gui-Lin CHEN. In vitro Germination,Callus Induction and Primary Haustorium Organogenesis in the Parasitic Plant Cynomorium songaricum[J]. Bulletin of Botanical Research, 2020, 40(6): 846-854.

图/表 5

参考文献 36

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Treatment	Plant growth regulator(mg·mL^-1)			Rate of callus induction(%)
Treatment	2,4-D	KT	GA₃	Rate of callus induction(%)
1	0.5	0.1	0.5	1.7±1.5^f
2	1.0	0.5	1.0	13.7±3.1^a
3	2.0	1.0	2.0	9.0±1.0^b
4	1.0	0.1	2.0	5.3±1.5^d
5	2.0	0.5	0.5	2.7±1.2^ef
6	0.5	1.0	1.0	7.3±1.5^c
7	2.0	0.1	1.0	3.3±1.5^e
8	0.5	0.5	2.0	6.0±1.7^cd
9	1.0	1.0	0.5	2.3±0.6^ef

Treatment	Plant growth regulators(mg·mL^-1)		Frequency of haustorium formation(%)	Number ofhaustorium
Treatment	2.4-D	KT	Frequency of haustorium formation(%)	Number ofhaustorium
1	0.00	0.25	0.0±0.0^b	0.0±0.0^c
2	0.25	0.25	33.3±23.1^b	3.7±0.6^b
3	0.50	0.25	66.7±30.6^a	5.3±2.5^ab
4	0.75	0.25	13.3±11.5^b	6.7±2.1^ab
5	1.00	0.25	16.7±5.8^b	8.3±1.5^a

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寄生植物锁阳种子萌发方法及愈伤组织、初生吸器诱导研究

In vitro Germination,Callus Induction and Primary Haustorium Organogenesis in the Parasitic Plant Cynomorium songaricum

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