%0 Journal Article
%A LI Ji-Lin
%A LI Xin-Ling
%A QU Min
%A XU Xiang-Ling*
%T Chitinase gene mediated by Ri plasmid transfering wheat
%D 2006
%R 10.7525/j.issn.1673-5102.2006.03.011
%J Bulletin of Botanical Research
%P 297-301
%V 26
%N 3
%X Mediated by <em>Agrobacterium rhizogene</em> which contains plasmid pMLH7133-Chi, the genetic transformation experiment was carried out with the callus of Spring Wheat Dongnong 7742, Longmai 9814, and Hite as the acceptor in the way of orthogonal test of L<sub>9</sub>(3<sup>4</sup>). The result indicates that the best group is Dongnong 7742, 100 &mu;m of Acetosyringone, <em>A. rhizogene</em>&rsquo;s OD<sub>600</sub>=0.8 and about two days&rsquo; co-cultivation; the order of influential factors is: genotype&gt;concentration of Acetosyringone&gt;concentration of <em>A. rhizogene</em>&gt; co-culture period. 138 regenerated plants were obtained in the experiment. Then detected by PCR amplification and PCR-Southern, the result suggests that the foreign Chitinase genes have already been introduced into 5 wheat genomes of all. Three of the five plants were detected again by RT-PCR and Chitinase activity methods, which proved that the foreign Chitinase gene has expressed steadily in wheat genome.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2006.03.011