%0 Journal Article
%A AN Zhi-Gang*
%A LIU Da-Li
%A LU Zhen-Qiang
%A MA Long-Biao
%A MAO Zi-Jun
%T Enhanced Heavy Metal Tolerance and Accumulation of E.coli by Overexpressed AtGCS from Arabidopsis thaliana
%D 2013
%R 10.7525/j.issn.1673-5102.2013.03.012
%J Bulletin of Botanical Research
%P 325-329
%V 33
%N 3
%X GSH is one of the most ubiquitous proteins known to provide protection against toxic heavy metals. A gene-AtGCS encoding Arabidopsis γ-glutamylcysteine synthetase was introduced into E.coli (BL21) by over-expression of TrxA-AtGCS fusion protein to analyze the capacity to enhance heavy metal tolerance of transformed E.coli. As a comparison, strain over-expressing only TrxA was used as a control. The results showed that the growth of E.coli cells by over-expressing TrxA-AtGCS was much better than the control cells expressing TrxA under 1 mmol·L-1 Cd2+, Zn2+ and Cu2+ heavy metal stresses. Meanwhile, more than 5 times’ bioaccumulation of Cd2+, Zn2+, Cu2+ and more than 4 times’ GSH content were observed in the strain over-expressing TrxA-AtGCS relatively to control cells, especially under cadmium ions with 10 times’ accumulation in cells over-expressing TrxA-AtGCS. It could be concluded that over-expression of AtGCS offered a promising heavy metal resistance of E.coli with superior heavy metal accumulation and GSH content.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2013.03.012