%0 Journal Article
%A HONG Sen-Rong*
%A YIN Ming-Hua
%T Preliminary Study on In vitro Conservation of Dioscorea bulbifera L. Virus-free Plantlet Germplasm and its Genetic Stability and Virus Change
%D 2011
%R 10.7525/j.issn.1673-5102.2011.05.012
%J Bulletin of Botanical Research
%P 579-584
%V 31
%N 5
%X The effect of inorganic salts level, sucrose, mannitol and plant growth inhibitor PP333 on In vitro conservation of Dioscorea bulbifera L. virus-free plantlet stems with a bud in MS basic medium was studied using single-factor experiments and orthogonal experimental method. The results showed that the survival rate of D.bulbifera L. virus-free plantlet stems with a bud which cultured on 1/4MS medium containing 2.0 mg·L-1 KT, 0.5 mg·L-1 NAA, 30 g·L-1 sucrose, 20 g·L-1 mannitol and 4 mg·L-1 PP333 and stored at 25±1℃ temperature was over 90%. After morphological indexes measurement, physiological and biochemical indexes determination and RT-PCR analysis, the genetic variation and PVY of In vitro conservation D.bulbifera L. virus-free plantlets were not detected. The results provided a simple and effective way for In vitro conservation of D.bulbifera L. virus-free plantlets germplasm.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2011.05.012