%0 Journal Article
%A HU Xiu-Feng
%A LI Yan-Fang
%A SHI Xiao-Gang
%A WANG Zhong-Kang*
%A YIN You-Ping
%T Cloning and Expression Analysis of the <em>CsMYB</em> Gene from <em>Citrus sinensis </em>Infected by <em>Ca</em>. Liberibacter asiaticus
%D 2011
%R 10.7525/j.issn.1673-5102.2011.05.006
%J Bulletin of Botanical Research
%P 543-549
%V 31
%N 5
%X In the present study, according to the results of <em>Citrus sinensis</em> SSH library analysis, primers of one up-regulation expressed EST sequence were designed, a MYB type gene was cloned by rapid amplification of cDNA ends (RACE). This gene was named as <em>CsMYB</em>, and submitted in GenBank (accession No. HQ841074). The full-length cDNA sequence of <em>CsMYB</em> is 1 306 bp, including an open reading frame (ORF) of 909 bp and the typical 26 bp poly-A. Bioinformatics analysis showed that the gene putatively encodes a protein which has 302 amino acids with molecular weight 32.97 kD, and its theoretical pI is 8.5. The <em>CsMYB</em> gene contains two typical conserved motifs: R2 and R3. The gene expression profile under the treatment of <em>Ca</em>. Liberibacter asiaticus(Las) was investigated by Real-time qPCR. The results showed that <em>CsMYB</em> gene expression varied at different times infected by <em>Las</em>, and was different in huanglongbing development progresses. Therefore, the <em>CsMYB</em> gene is speculated to be a transcription factor and possibly to be involved in the procedure induced defense of <em>Las</em>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2011.05.006