%0 Journal Article
%A Boyan LIU
%A Fengjin ZHU
%A Renxiang CHE
%A Xingguo LAN
%A Yukun ZHANG
%A Yutong LU
%T Identification of <i>OsBBTIs</i> Gene Family in Rice and Its Expression Analysis under Abiotic Stress
%D 2025
%R 10.7525/j.issn.1673-5102.2025.05.009
%J Bulletin of Botanical Research
%P 745-754
%V 45
%N 5
%X <p>Bowman-Birk trypsin inhibitor（BBTI） is a widely distributed trypsin inhibitor in plants， which plays an important role in the process of plant resistance to the stresses. In this study， 11 rice <i>BBTIs</i> gene family members were identified at the genome level by bioinformatics methods， among which 10 <i>OsBBTI</i> genes were clustered at the front of chromosome 1， and <i>OsBBTI13</i> gene was located on chromosome 3. OsBBTIs protein had N-terminal signal peptide and contained 1-3 typical conserved Bowman-Birk type domains. The promoter region of <i>OsBBTIs</i> gene had response elements related to plant hormones jasmonic acid， abscisic acid and the abiotic stresses. Transcriptome data showed that all <i>OsBBTIs</i> gene can be induced by jasmonic acid， and most <i>OsBBTI</i> genes can be expressed in response to the abiotic stresses. qRT-PCR was used to analyze the expression level of <i>OsBBTIs</i> gene under NaHCO<sub>3</sub> and NaCl stresses， the results showed that the expression level of <i>OsBBTIs</i> gene increased under the saline-alkali stresses. This study laid a foundation for further study on the biological function of OsBBTIs proteins in abiotic stress.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2025.05.009