%0 Journal Article
%A Baodong CHEN
%A Ning GONG
%A Qiong WANG
%A Wei LIU
%A Wu LIU
%A Yaying YANG
%A Yi WANG
%A Ziwei ZOU
%T Growth and Physiological Responses of Ancient <i>Celtis sinensis</i> Seedlings to the Inoculation of Indigenous Arbuscular Mycorrhizal Fungi
%D 2025
%R 10.7525/j.issn.1673-5102.2025.03.010
%J Bulletin of Botanical Research
%P 393-405
%V 45
%N 3
%X <p>Ancient trees in urban areas are affected by their own and environmental factors， resulting in growth restriction. Inoculation with indigenous dominant arbuscular mycorrhizal（AM） fungi in the rhizosphere soil of ancient trees may be a new method of ancient tree rejuvenation. Two indigenous AM fungal mycorrhizal agents（<i>Claroideoglomus lamellosum</i> and <i>Funneliformis mosseae</i>） were screened and propogated from the rhizosphere soil of <i>Celtis sinensis</i> trees in Shanghai， and were verified by inoculation into pots of <i>C. sinensis</i> seedlings. The results showed that plant height， stem diameter and leaf area of the inoculated seedlings increased significantly compared to the control， and the number of leaves also increased significantly. The mixed inoculation had a significant effect on the biomass of the whole plant and number of root tips. The accumulation of nitrogen（N） and phosphorus（P） nutrients， photosynthetic capacity and chlorophyll content of the seedlings were improved as compared with that of the control， respectively. Inoculation with both strains enhanced the activities of superoxide dismutase （SOD）， peroxidase （POD）， and catalase （CAT） in leaves and enhanced the resistance of live seedlings. Based on a comprehensive evaluation of the fuzzy membership function， an optimal inoculation strategy for promoting the growth of ancient <i>C. sinensis</i> seedlings was identified as FM+CL. This work provided a valuable reference for the application of indigenous AM fungi agents in the rejuvenation of ancient trees.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2025.03.010