To explore the pollen morphological characteristics of Nymphaea and their taxonomic significance， the pollen of 113 species or cultivars from five subgenera and two trans-subgeneric hybrids of Nymphaea were used as materials and their pollen morphological characters were observed by scanning electron microscope. The results showed that： （1）The pollen of Nymphaea were all single grains， radially symmetric， and medium size（10.05-37.04） μm×（21.96-64.83） μm. （2）Among them， the pollen germination troughs of Nymphaea in 86 samples were zonoporate， 23 samples were anazonoporate， and 4 samples were anacolpate. （3）The pollen exine ornamentation included smooth， micro verrucous， rough， nodular， baculate， and caveola type. （4）The 113 samples of Nymphaea germplasms were clustered into five groups， of which the subgenus Nymphaea was characterized primarily by anazonoporate germination trough and nodular and baculate exine ornamentation， distinguished it from other subgenera. In conclusion， there were some differences in pollen size， germination trough type， and exine ornamentation among different subgenera and trans-subgeneric hybrids of Nymphaea， which might serve as auxiliary reference indicators for classification and provided palynological evidence for the study of genetic and phylogenetic relationship of Nymphaea.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2025.02.014