%0 Journal Article
%A Chunming LI
%A Haijuan FAN
%A Haixia LI
%A Huanzhen LIU
%A Jun WU
%A Linping TIAN
%A Wenzhe HAN
%A Yu QI
%A Yuandong MA
%A Zhaoning WANG
%T Stumping Effect on Rejuvenation of Twigs and Rooting of Softwood Cuttings of <i>Acer Mandshuricum</i>
%D 2025
%R 10.7525/j.issn.1673-5102.2025.02.009
%J Bulletin of Botanical Research
%P 228-240
%V 45
%N 2
%X <p><i>Acer mandshuricum</i> is a colourful leafy tree species with great ornamental value in Northeast China， but it is difficult to reproduce. The aim of this study was to improve the rooting rate of <i>A. mandshuricum</i> softwood cuttings and to clarify the relationship between stumping treatment and rooting of softwood cuttings. In 2023， the cross-sectional anatomical structure and growth index of twigs， the physiological and biochemical index of leaves， and the rooting index of softwood cuttings of Pc-2（stumped in 2022）， Pc-1（stumped in 2023） were observed， using the twigs of <i>A. mandshuricum</i> without stumping（Pc-CK） as a control. The results showed that： the rooting rate， average root length， average number of roots， and root effect index of Pc-1 softwood cuttings were higher than those of Pc-2（<i>P</i>&lt;0.05） and Pc-CK（<i>P</i>&lt;0.05）. The thickness of the epidermal and cortical collenchyma and the thickness of the xylem in the cross-section of their twigs from small to large were Pc-1， Pc-2 and Pc-CK（<i>P</i>&lt;0.05）. The length， basal diameter， leaf area， leaf fresh weight， and water content of Pc-1 twigs were significantly higher than those of Pc-CK（<i>P&lt;</i>0.05）. The net photosynthetic rate and stomatal conductance of Pc-1 leaves were significantly increased by 90% and 89%（<i>P&lt;</i>0.05） respectively， compared to Pc-CK. The contents of chlorophyll a， carotenoid， soluble sugar， and soluble protein， as well as the activities of superoxide dismutase（SOD）， peroxidase（POD）， and catalase（CAT） in Pc-1 leaves were significantly higher than those of Pc-CK（<i>P&lt;</i>0.05）. The contents of malondialdehyde（MDA）， total flavonoids， and total phenolics in Pc-CK leaves were significantly higher than those in Pc-1（<i>P&lt;</i>0.05）. In conclusion， the stumping treatment made the twigs of <i>A. mandshuricum</i> rejuvenated， which promoted the rooting from softwood cuttings. The Pc-1 twigs showed better juvenile characteristics and rooting effect on softwood cuttings， which provided support for the softwood cutting propagation of <i>A. mandshuricum.</i></p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2025.02.009