%0 Journal Article
%A Anqi WANG
%A Hanqing FENG
%A Honghong YANG
%A Ji ZHANG
%A Jianping WU
%A Kun SUN
%A Min SUN
%A Xiaowei DA
%A Yuejing ZHANG
%T Effects of Different Vectors and <i>Agrobacterium</i><i>tumefaciens</i> on Transient Expression of Alfalfa
%D 2023
%R 10.7525/j.issn.1673-5102.2023.06.006
%J Bulletin of Botanical Research
%P 835-845
%V 43
%N 6
%X <p>Alfalfa（<i>Medicago sativa</i>） is an important forage plants. In order to effectively express foreign genes in alfalfa， by using the green fluorescent protein（GFP） as report gene and <i>Nicotiana benthamiana</i> as a comparison， the effects of two different vectors（the non-replicating and the BeYDV（bean yellow dwarf virus）-based replicating vectors）， and two different <i>Agrobacterium tumefaciens</i> strains（LBA4404 and EHA105） on the transient expression of alfalfa leaves were investigated. The results showed that the combination of LBA4404 with the replicating vector（re-vector/LBA4404） led to effective expression of GFP in alfalfa leaves. By using LBA4404 with the replicating vector， the effects of the concentration of<italic/>re-vector/LBA4404 and incubation time after infection on the transient expression were also evaluated. It was found that with the increase of concentration of bacterial suspension or time post-infection， the expression level of GFP increased first but then decreased. In the leaves of alfalfa， the highest expression level of GFP was observed when the concentration of LBA4404 strain was 1.0（OD<sub>600</sub>）； and the expression level of GFP reached the peak at 5-7 d after infection. Compared with <i>Nicotiana benthamiana</i>， achieving the highest expression level of GFP in alfalfa might need higher concentration of <i>Agrobacterium tumefaciens</i> and longer incubation time after infection.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2023.06.006