%0 Journal Article
%A Jiale LI
%A Meiling XIA
%A Qingqing HUO
%A Shuya ZHANG
%A Wei HAN
%A Wenhua GUO
%A Xiangling YOU
%A Yuxin XIA
%A Zhe ZHANG
%T Effect of Expression of <i>Aeβ</i>-<i>AS</i> gene from <i>Aralia elata</i> on the Content of Saponins in <i>Nicotiana tabacum</i> L.
%D 2023
%R 10.7525/j.issn.1673-5102.2023.05.014
%J Bulletin of Botanical Research
%P 779-786
%V 43
%N 5
%X <p>To investigate the effect of <i>β</i>-amyrin synthase gene（<i>Aeβ</i>-<i>AS</i>） on triterpenoid saponin synthesis of <i>Aralia elata</i>， the <i>Aeβ</i>-<i>AS</i> gene was cloned and genetically transformed into tobacco. The expression difference of <i>Aeβ</i>-<i>AS</i> gene in different parts of transgenic tobacco was analyzed， and the expression levels of <i>Aeβ</i>-<i>AS</i> gene， upstream and downstream key enzyme genes and total triterpene content were detected respectively. The results showed that the plant overexpression vector PROKⅡ-<i>Aeβ</i>-<i>AS</i> was successfully constructed and transferred into wild type tobacco， and seven transgenic lines were obtained and expressed at the mRNA level， and the expression level in leaves was higher than that in roots and stems. In transgenic tobacco， the expression levels of <i>Aeβ</i>-<i>AS</i> gene and its upstream and downstream key enzyme genes were higher than those of wild type， and the relative expression levels of <i>NtFPS</i> and <i>NtSS</i> genes of strain L21 were the highest， and the relative expression levels of <i>NtSE</i> and <i>Aeβ</i>-<i>AS</i> genes of strain L30 were the highest. Compared with wild-type tobacco， the total triterpene content of GM tobacco was significantly increased（1.1-1.6 times）. The results showed that synthesis of <i>Aeβ</i>-<i>AS</i> gene and heterologous transformation in tobacco could significantly increase the total triterpene content in transgenic tobacco.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2023.05.014