%0 Journal Article
%A Bingbing GUO
%A Hong YANG
%A Lifeng WANG
%A Longjun DAI
%T Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree (<i>Hevea brasiliensis</i>)
%D 2023
%R 10.7525/j.issn.1673-5102.2023.01.008
%J Bulletin of Botanical Research
%P 69-75
%V 43
%N 1
%X <p>In order to elucidate the mechanism of tapping panel dryness（TPD） and effectively control it， the ultrastructure and the expression patterns of reactive oxygen species（ROS）-related genes in barks from healthy（H） and different degrees of TPD trees were studied in this study. The results showed that with the development of TPD， aberrant mitochondrial ultrastructure changed including the loss of the matrix， swelling of cristae， and the cavitation of the inner cavity were observed in barks. The results of qRT-PCR experiments showed that the expression levels of <i>HbPOD2</i> and <i>HbPOD3</i> in TPD trees were higher than those in healthy trees， indicating the possibility of these genes being used as “marker” genes to monitor the tapping intensity， the stimulation intensity， and the occurrence of TPD. The gene <i>HbCAT</i> was also down-regulated in TPD trees， indicating that the balance between ROS generation and scavenging may be a key factor in the occurrence of TPD in rubber tree. The expressions of <i>HbGST1</i>， <i>HbGST2</i>， and <i>HbPPO</i> in TPD trees were higher than those in healthy trees， indicating these genes may be associated with <i>in</i>-<i>situ</i> coagulation of latex during TPD occurrence. This study reveals the changes of ultrastructure and the expression patterns of ROS-related genes in rubber tree barks during TPD occurrence. These findings may provide a new perspective for elucidating the mechanism of TPD in rubber tree， and provid theoretical guidance for developing methods for monitoring tapping intensity and stimulation intensity and for developing TPD-related “marker” genes in rubber trees.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2023.01.008