%0 Journal Article %A Chunguang BAO %A Fenglan HUANG %A Huibo ZHAO %A Qi WEN %A Ruxin LI %A Zhiqiang ZHAO %T Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress %D 2023 %R 10.7525/j.issn.1673-5102.2023.01.005 %J Bulletin of Botanical Research %P 36-50 %V 43 %N 1 %X

In order to reveal the mechanism of Ricinus communis response to heavy metal cadmium(Cd) stress, and screen out the resistance genes involved in Cd stress, by observing the seed germination and plant growth status, castor plants treated with water were used as controls to study their effects under three doses(300, 700 and 1 000 mg·L-1) of Cd stress. The response mechanism is expected to provide new ideas for revealing the defense and detoxification mechanism of castor plant under in Cd stress. Using differential proteomics to analyze the network regulation mechanism of castor under Cd stress, that is, with the increase of Cd stress concentration, the castor plant blocks the absorption of heavy metal Cd by roots, improves their own antioxidant capacity, and inhibits Cd2+ operation. As well as the induction of programmed cell death and other detoxification processes to resist Cd stress damage; according to the results of omics analysis, a significantly different gene RcBSK7 was screened. Through functional verification in Arabidopsis, it can be seen that this gene can improve tolerance of castor plants to Cd stress. This study enhances the understanding of the diversity and complexity of castor plants under three Cd stresses, and provides a valuable theoretical basis for the identification of Cd-tolerant genes and the restoration of heavy metal pollution in the soil.

%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2023.01.005