%0 Journal Article
%A Bi QIN
%A Fei HUANG
%A Lifeng WANG
%A Meng WANG
%A Mingyang LIU
%T Cloning and Expression Analysis of <i>DELLA</i> gene <i>HbRGL1</i> from <i>Hevea brasiliensis</i> Müll. Arg
%D 2022
%R 10.7525/j.issn.1673-5102.2022.06.009
%J Bulletin of Botanical Research
%P 997-1004
%V 42
%N 6
%X <p>DELLA protein belongs to the plant-specific GRAS family and is a negative regulator of plant growth. To reveal the molecular regulation mechanism of <i>DELLA</i> gene in rubber tree growth and development， the full-length cDNA sequence of <i>HbRGL1</i> was cloned from rubber tree cultivar Reyan 7-33-97. The ORF of <i>HbRGL1</i> was 1 851 bp， and encoded 616 amino acids. Bioinformatics analysis showed that HbRGL1 was an unstable hydrophilic protein containing two conserved domains of DELLA and GRAS， and localized in the nucleus. Phylogenetic analysis showed that HbRGL1 was clustered with HbGAIL of rubber tree， MeGAIL of <i>Manihot esculenta</i>， RcGAI of <i>Ricinus communis</i>， and JcGAI<italic/>of <i>Jatropha curcas</i>. qRT-PCR analysis showed that the expression level of <i>HbRGL1</i> in different tissues of rubber tree<italic/>was significantly different， and its expression level was the highest in flower. <i>HbRGL1</i> expression was significantly up-regulated in leaves treated by different hormones such as auxin（IAA）， ethephon（ET） and abscisic acid（ABA）. Especially， and it rapidly responded to GA<sub>3</sub> treatments， and had the highest up-regulation of expression level. The study provided theoretical basis for further elucidating the function of <i>HbRGL1</i> in the growth and development of rubber tree.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.06.009