%0 Journal Article
%A Chenxu LIU
%A Liran YUE
%A Yingjie LIU
%A Yunwei ZHOU
%T Cloning and Functional Analysis of miR398a from <i>Chrysanthemum</i>× <i>grandiflora</i> in Response to Salt Stress
%D 2022
%R 10.7525/j.issn.1673-5102.2022.06.008
%J Bulletin of Botanical Research
%P 986-996
%V 42
%N 6
%X <p>miRNAs played an important role in abiotic stress， and miR398a and pre-miR398a sequences were obtained from Small RNA database of <i>Chrysanthemum×grandiflora</i>. The sequence alignment showed that the cgr-miR398a was highly conserved with the identified miR398a sequences of other plants. Target genes prediction of cgr-miR398a showed that their annotation of targets include superoxide dismutase［Cu-Zn］（CSD2）， copper chaperone（CCS）， A20/AN1-like zinc finger family protein（SAP8）. The qPCR assay showed that the expression levels of miR398a was significantly decreased while target genes showed contrary trend during salt stress in <i>Chrysanthemum×grandiflora.</i> To explore the function of cgr-mir398a in response to salt stress， and an overexpression vector of cgr-mir398a was constructed and transformed<italic/>into <i>Arabidopsis thaliana.</i>The result showed that overexpression of the cgr-MIR398a in <i>Arabidopsis</i> could decrease seed germination under salt stress and salt tolerance at the adult seedling stage， therefore cgr-miR398a played a negative regulatory role in <i>Arabidopsis</i> in response to salt stress. The results provided a valuable reference for further exploring the functional mechanism of miR398a during salt stress in <i>Chrysanthemum×grandiflora</i>.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.06.008