%0 Journal Article
%A Anying HUANG
%A Dean XIA
%A Dongchen NA
%A Qing YAN
%A Yang ZHANG
%A Zhigang WEI
%T Cloning and Drought Tolerance Expression Analysis of <i>PtrWRKY51</i> Gene in <i>Populus trichocarpa</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.06.010
%J Bulletin of Botanical Research
%P 1005-1013
%V 42
%N 6
%X <p>In order to clarify the function of <i>PtrWRKY51</i> gene of <i>Populus trichocarpa</i>， the CDS of <i>PtrWRKY51</i> gene was cloned using <i>P. trichocarpa</i> from Nisqually-1 strain as material. The function of <i>PtrWRKY51</i> gene was studied by bioinformatics analysis， and assayed by yeast self-activation verification， and subcellular localization and simulated drought stress by real-time qPCR. The results showed that the CDS of <i>PtrWRKY51</i> was 579 bp and encoded 192 aa. Bioinformatics analysis and subcellular localization experiments showed that PtrWRKY51 was a non-transmembrane alkaline unstable hydrophilic protein， located in the nucleus and contained a conserved domain unique to <i>WRKY</i> family， it was a <i>WRKY</i> transcription factor classified to class IIc. The yeast self-activation verification experiment showed that <i>PtrWRKY51</i> gene could self-activation activity. qRT-PCR showed that the expression of this gene was significantly induced by 8% PEG6000 and reached the highest expression in stem and leaf after 12 h stress， while the roots appeared after 24 h stress. This study could provide reference for further study on stress tolerance and biological function of <i>PtrWRKY51</i>.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.06.010