%0 Journal Article
%A Denggao LI
%A Na ZHOU
%A Qinghui MU
%A Rui LIN
%A Wei BAI
%A Yanru ZHANG
%T Cloning and Functional Analysis of <i>StNPR4</i> gene in <i>Solanum tuberosum</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.05.013
%J Bulletin of Botanical Research
%P 821-829
%V 42
%N 5
%X <p>In order to clarify the function of <i>StNPR4</i> in potato， the CDS and promoter sequence of <i>StNPR4</i> were cloned and bioinformatics analysis was conducted. Tissue expression pattern was performed by qRT-PCR， and a binary expression vector of <i>StNPR4</i> driven by its own promoter was constructed and transformed into potato. Transgenic plants were used to examine the responses to salicylic acid， <i>Phytophthora infestans</i> and high salt stress respectively. The results showed that StNPR4 had typical functional domains in NPR1 family with cis-acting elements on the promoter in response to biotic and abiotic stresses. The <i>StNPR4</i> expression level was highest in leaves <i>StNPR4</i> was inductively expressed by SA and the induced expression was higher in transgenic plants than in the control. <i>StNPR4</i> transgenic potato plants showed enhanced resistance to <i>Ph. infestans</i> and higher rooting rate under high salt stress. This indicated that <i>StNPR4</i> played an important role not only in biotic stress but also in abiotic stress of potato.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.05.013