%0 Journal Article
%A Bingyan SHAN
%A Ermei CHANG
%A Hongli LI
%A Jianfeng LIU
%A Xiulian ZHAO
%A Yuening HUANG
%A Zeping JIANG
%T Comparison of Genetic Diversity Between Wild and <i>ex</i>-<i>situ</i> Conservation Populations of <i>Cupressus chengiana</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.05.008
%J Bulletin of Botanical Research
%P 772-779
%V 42
%N 5
%X <p>To evaluate the genetic diversity level and provide theoretical basis of <i>ex</i>-<i>situ</i> conservation populations of <i>Cupressus chengiana</i>， the genetic diversity， genetic structure， and gene flow of <i>ex</i>-<i>situ</i> conservation populations and wild populations of <i>C. chengiana</i> were studied respectively. The principal component analysis（PCA）， cluster analysis， construction of molecular evolutionary tree， genetic diversity and genetic structure of three wild populations and <i>ex</i>-<i>situ</i> conservation populations based on SNP locus were obtained respectively from Genotyping-by-Sequencing technology（GBS） sequencing. The results showed that high quality clean data 118 321 514 728 bp was obtained by GBS sequencing， and 1 947 047 tags were developed. After being screened， a total of 1 259 610 of high-quality SNP sites were retained. The valley value of cross validation error rate determined that the optimal number of clusters was 1. The phylogenetic tree indicated that most of individuals <i>ex</i>-<i>situ</i> conservation populations and wild populations were clustered together. Both of combined <i>ex</i>-<i>situ</i> conservation populations and wild populations of <i>C. chengiana</i> had higher genetic diversity， and their value of heterozygosity（<i>H</i><sub>o</sub>）， expected heterozygosity（<i>H</i><sub>e</sub>）， Shannon information index（<i>S</i><sub>hi</sub>）， inbreeding coefficient（<i>F</i><sub>is</sub>）， and percentage of polymorphic loci（<i>P</i><sub>ic</sub>） were 0.181 5-0.272 0， 0.223 2-0.300 3， 0.331 0-0.464 9， 0.178 0-0.246 5 and 0.272 2-0.309 2， respectively. Genetic diversity of <i>ex</i>-<i>situ</i> conservation cultivated populations was relatively higher than the wild populations of <i>C. chengiana</i>， the value of <i>H</i><sub>e</sub> and <i>S</i><sub>hi</sub> was 0.300 3 and 0.464 9， respectively. The wild populations<italic/>of <i>C. chengiana</i> of Baiwan tunnel（BW）_vs_Songgang town（SA） had the greater genetic differentiation and the gene flow（<i>N</i><sub>m</sub>） were lower than other populations（<i>F</i><sub>st</sub>=0.091，<i>N</i><sub>m</sub>=2.496）. The results indicated that genetic differentiation had not occurred among these <i>C. chengiana</i> populations， and the gene had exchanges frequently among the <i>ex</i>-<i>situ</i> conservation populations and wild populations of <i>C. chengiana</i>（<i>F</i><sub>st</sub>&lt;0.05， <i>N</i><sub>m</sub>&gt;4）. There was a close genetic relationship between the <i>ex</i>-<i>situ</i> conservation individuals and the wild populations individuals， and there was no obvious clustering phenomenon. Thus， transplanting endangered plants was a better method in <i>ex</i>-<i>situ</i> conservation. This paper would provide a reference for the <i>ex</i>-<i>situ</i> conservation populations of wild populations <i>C. chengiana</i> and provided a theoretical reference for the conservation of other tree germplasm resources.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.05.008