%0 Journal Article
%A Gonggui FANG
%A Huaizhi MU
%A Jing JIANG
%A Kun CHEN
%T Analysis of the Promoter Sequence and Response Characteristics of the <i>BpPIN3</i> gene in <i>Betula platyphylla</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.04.009
%J Bulletin of Botanical Research
%P 592-601
%V 42
%N 4
%X <p>As an important auxin efflux carrier family in plants， PIN protein family shows extensive physiological effects in plant growth and development. In order to further understand the function of <i>BpPIN3</i> and explore its response to different hormone signals and abiotic stresses during <i>Betula platyphylla</i> development，the <i>BpPIN3</i> promoter sequence was analyzed by bioinformatics methods. Tissue expression patterns of roots， stems， leaves and apical buds of annual and biennial <i>B. platyphylla</i> clones were analyzed respectively. The seedlings were used as conduct hormone induction and light stress treatment with 100 μmol·L<sup>-1</sup> auxin<sup/>（IAA）， 100 μmol·L<sup>-1</sup> gibberellin（GA<sub>3</sub>）， 200 μmol·L<sup>-1</sup> abscisic acid（ABA） and long light conditions， respectively. RNA was extracted from leaves and roots of <i>B. platyphylla</i> at 0， 2， 4， 8， 16， 24 and 48 h after hormone treatment and at 0， 1， 3， 6， 12， 24， 48 and 72 h after light stress， and qRT-PCR was used to analyze the expression of <i>BpPIN3</i>. The results showed that the <i>BpPIN3</i> promoter sequence contained different types of auxin response elements such as gibberellin， abscisic acid， and methyl jasmonate， as well as multiple stress-related cis-regulatory elements. <i>BpPIN3</i> was expressed in many tissues of birch in different growth years， especially in leaves， and the expression of <i>BpPIN3</i> in the second year was higher than that in the first year. The relative expression of <i>BpPIN3</i> gene was different in different parts under different treatment conditions. IAA and GA<sub>3</sub> could induce the up-regulation of <i>BpPIN3</i> expression in leaf. Under ABA treatment， except for 16 and 48 h， the expression pattern of <i>BpPIN3</i> gene was opposite to that under IAA treatment. IAA， GA<sub>3</sub> and ABA could induce the expression of <i>BpPIN3</i> in root， but shading stress induced the expression of <i>BpPIN3</i> gene in leaf， the relative expression of <i>BpPIN3</i> gene was significantly higher than that of the control（0 h） from 12 h in root， IAA， GA<sub>3</sub> and ABA could induce the expression of <i>BpPIN3</i> in root， shading stress induced the expression of <i>BpPIN3</i> gene in leaf； the relative expression of <i>BpPIN3</i> gene was significantly higher than that of the control （0 h）from 12 h in root. The results suggested that <i>BpPIN3</i> gene played the important regulatory role in the growth and development of <i>B. platyphylla</i>，and the signal transduction pathways of IAA， GA<sub>3 </sub>and ABA and plant light response.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.04.009