%0 Journal Article
%A Jinbo Fu
%A Jing Yin
%A Xin Su
%A Yaguang Zhan
%A Yuqi Zhang
%A Zhiqiang You
%T 
%D 2022
%R 10.7525/j.issn.1673-5102.2022.02.014
%J Bulletin of Botanical Research
%P 289-298
%V 42
%N 2
%X <p>To explore a sustainable and stable way to obtain birch branches， a multi branch birch by promoting germination was established. One year old birch seedlings were used as experimental materials， and three cutting heights（10， 20 and 30 cm）and four hormone or chemical agents （20 mg·L<sup>-1</sup> 2，4-D， 20 mg·L<sup>-1</sup> KT， 20 mg·L<sup>-1</sup> 6-BA and 20 mg·L<sup>-1</sup> KMnO<sub>4</sub>） were used to promote the sprouting of birch seedlings. The results showed that the germination rate of birch seedlings was the highest， which reached 65.0% after the first cutting（30 d） and the cutting height was 10 cm. Under the treatment of sawdust moisture， the sprouting rate and sprouting yield of the three cutting heights were higher， the sprouting rate was more than 75.0%， and the yield was more than 77.0 g per plant. The content of total triterpenoids in the branches was the highest at 20 cm cutting height（76.43 mg·g<sup>-1</sup>）， followed by 10 cm cutting height. Other treatments were not conducive to the accumulation of total triterpenoids. The content of total triterpenoids in leaves was the highest under the treatment of 6-BA （91.16 mg·g<sup>-1</sup>）， which was more than 2.68 times of sawdust treatment（30 cm）. The content of total triterpenoids in the branches and leaves of sawdust， KT and KMnO<sub>4</sub> treatments was significantly lower than that of other treatments. 6-BA was beneficial to the accumulation of oleanolic acid in branches and leaves， while other treatments were not conducive to the accumulation of oleanolic acid. Sawdust treatment was beneficial to the secondary sprouting and yield improvement. The sprouting yield of the treatment with 10 cm cutting height reached 126.96 g per plant. The total triterpenoids content in the branches was 97.99 mg·g<sup>-1</sup> when treated with 6-BA at the cutting height of 30 cm. The content of total triterpenoids in leaves was option when the cutting height was 20 cm， reaching 99.22 mg·g<sup>-1</sup>， followed by sawdust treatment group. The results provid technical support for sustainable and efficient utilization of birch resources and obtaining triterpenoids.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.02.014