An efficient method was established for the separation and preparation of chlorogenic acid， pedaliin-6″-acetate and rosmarinic acid from Dracocephalum tanguticum Maxim.. The chlorogenic acid， pedaliin-6″-acetate and rosmarinic acid was enriched by semi-preparative high performance liquid chromatography（SP-HPLC） from ethyl acetate extract， and then separated and purification by high-speed counter-current chromatography（HSCCC） and preparative high performance liquid chromatography（Pre-HPLC）. A total of 54 mg of chlorogenic acid， 150 mg of pedaliin-6″- acetate and 200 mg of rosmarnic acid were obtained from ethyl acetate extract with purities of 96.9%， 97.9% and 95.1% by HPLC analysis， respectively. Their structures were identified by nuclear magnetic resonance spectrum. The result show that this method is suitable for the isolation and purification of chlorogenic acid， pedaliin-6″-acetate and rosmarnic acid from D. tanguticum. It also avoids the disadvantages of traditional separation methods such as many operations， large reagent consumption and non-recyclability， provides a reference basis for the study of separating of active components and preparation reference substance from D.tanguticum.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.02.010