As one of the three floral pathway integrators， FT（FLOWERING LOCUS T） and its homologous genes were considered to be important genes which related to flowering. In order to clarify the function of FT homologous genes and flowering mechanism of saffron（Crocus sativus L.）， the three reported FT homologous genes， named CsatFT1， CsatFT2 and CsatFT3， were isolated and analyzed. The gDNA contained the ORF with length of 835， 1 642 and 1 132 bp， respectively， and they all had 4 exons and 3 introns. cDNA sequences contained the length of 528， 525 and 540 bp ORF， which encoded 175， 174 and 179 amino acids， respectively. The phylogenetic analysis indicated that CsatFT1， CsatFT2 and CsatFT3 showed relatively close genetic distance with the monocotyledon Narcissus tazetta NtFT， Lilium longiflorum LlFT and Allium cepa AcFT1， respectively. qRT-PCR results indicated that the expression levels of CsatFT1， CsatFT2 and CsatFT3 were the highest in the leaves， followed by the lateral roots， and almost no expressed in daughter corms and primary roots at the early stage of replacement corm enlargement. At the late stage of replacement corm enlargement， the expression levels of CsatFT1， CsatFT2 and CsatFT3 were all high in daughter corms and were almost not detected in terminal buds. During indoor storage stage， the expression levels of CsatFT1， CsatFT2 and CsatFT3 were high in the capital， followed by the leaves， and almost undetectable in the petals and anthers. From the phenotypes of transgenic tobacco and transgenic Arabidopsis plants， the results showed that CsatFT1， CsatFT2 and CsatFT3 could promote the early flowering of plants.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.02.007