%0 Journal Article
%A Chao Wang
%A Jingdan Cao
%A Jingjing Shi
%A Qian Sun
%A Yaxuan Zhang
%A Yuhang Wu
%T Bioinformatic Analysis and Expression Pattern of LTP Family Genes in <i>Populus davidiana</i> × <i>P. alba</i> var. <i>pyramidalis</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.02.006
%J Bulletin of Botanical Research
%P 211-223
%V 42
%N 2
%X <p>To lay a foundation for studying the molecular regulation mechanism of <i>LTP</i> family gene and molecular genetic improvement of <i>Populus davidiana × P. alba</i> var. <i>pyramidalis</i>， the expression pattern of <i>LTP</i> family genes from <i>P. davidiana × P. alba</i> var. <i>pyramidalis</i> was analyzed.The protein properties， multiple sequence alignments and phylogenetic tree analysis of LTP family members were performed to show their structural features. The <i>P. davidiana × P. alba</i> var. <i>pyramidalis</i> seedlings treated with gravity， salt and PEG stress respectively were subjected to quantitive real time PCR analysis. The expression patterns of <i>PdbLTP</i> family genes were carried out respectively. Eight <i>PdbLTP</i> genes and two subfamily <i>PdbGLTP</i> genes were obtained， and their CDS lengths were ranged from 294-396 bp. LTP family were hydrophobic proteins and had eight cysteine conserved structures， without transmembrane region. qRT-PCR analysis showed that the expression patterns of different<i> PdbLTP</i> family genes were different under gravity processing. Expression levels of <i>PdbLTP1</i>， <i>PdbLTP3</i>， <i>PdbLTP5</i> and <i>PdbLTP7 </i>genes were up-regulated in tension wood compared to those in the normal wood. The transcripts levels of <i>PdbLTP1</i>， <i>PdbLTP2</i>， <i>PdbLTP3</i>， <i>PdbLTP5 </i>genes were abundant in stem.<italic/>Expression of <i>PdbLTP</i> family genes was induced by salt stress except for <i>PdbLTP5. PdbLTP1</i>， <i>PdbLTP2</i>， <i>PdbLTP3</i> and <i>PdbLTP5 </i>were up-regulated under drought stress<i>.</i> Different members of <i>PdbLTP</i> gene family play important roles in regulation of xylem development and tolerance to abiotic stress in <i>Populus davidiana × P. alba</i> var. <i>pyramidalis</i>.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.02.006