%0 Journal Article
%A Hailong Pang
%A Hanqing Feng
%A Juanjuan Wang
%A Lingyun Jia
%A Ying Li
%A Yuejing Zhang
%T Effects of Three Kinds of <i>Agrobacterium</i> and Different Transformation Conditions on the Transient Expression of GFP in <i>Nicotiana benthamiana</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.01.013
%J Bulletin of Botanical Research
%P 121-129
%V 42
%N 1
%X <p>By using <i>Nicotiana benthamiana</i> as plant material， the influence of the different <i>Agrobacterium</i> strains（LBA4404， EHA105， GV3101）， bacterial concentration and infection time on GFP（as the reporter gene） the fluorescence transient expression after transformation were analyzed respectively. The results showed that the expression of GFP with different <i>Agrobacterium</i> strains were various in the optimal concentration and time for transient： the highest transient expression efficiency with LBA4404 strain was achieved when the OD<sub>600</sub> of bacterial suspension was 0.8； while the highest transient expression efficiency with EHA105 and GV3101 strains was achieved when the OD<sub>600</sub> of the bacterial suspension reached 0.6. The level of transient expression by strain LBA4404 was the highest at 2 days later <i>Agrobacterium</i> infiltration while the level of transient expression by strains EHA105 and GV3101 was the highest at 4 days later <i>Agrobacterium</i> infiltration. The comparative analysis among these different strains showed that the transient expression efficiency by using LBA4404 strain was the highest. The above results indicated that <i>Agrobacterium</i> strains and transformation conditions such as bacterial concentration and infection time were important factors that affected the transient expression efficiency.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.01.013