%0 Journal Article
%A Chang Qu
%A Guifeng Liu
%A Jing Jiang
%A Yang Wang
%A Yunli Yang
%T Tissue-specific Expression and Analysis of Exogenous Hormone Response of <i>BpPIN</i>5 Gene Promoter in <i>Betula platyphylla</i>
%D 2022
%R 10.7525/j.issn.1673-5102.2022.01.011
%J Bulletin of Botanical Research
%P 104-111
%V 42
%N 1
%X <p>Being the polar carrier of IAA， PIN family proteins played a key role in plant embryonic development， organ development and tropic growth， especially in the formation of plant phyllodes， veins and vascular tissue differentiation.In order to identify the response characteristics <i>BpPIN</i>5 gene to exogenous hormones， the upstream 1447 bp sequence of <i>BpPIN</i>5 was cloned from the genome DNA of <i>Betula platyphylla</i>. The cis-acting elements in the sequence were predicted by PLACE online software. The results showed that the promoter sequence of <i>BpPIN</i>5 contained different types of auxin responsive elements such as auxin， gibberellin， Salicylic acid， abscisic acid， methyl jasmonate， ethylene respectively. Transgenic vector pro-BpPIN5：：GUS to <i>B. platyphylla </i>was constructed. GUS histochemical staining analysis reflicted that <i>BpPIN</i>5 promoter had transcriptional activity in the tip of leaf crack， venule and root of <i>B. platyphylla</i> respectively. After transgenic <i>Betula platyphylla</i> was treated with IAA， GA， MeJA， SA and ABA respectively， the results showed that <i>BpPIN</i>5 promoter responded to the five hormones above at the crack edge of the first leaf， petiole and root tissue of the second leaf， and the response changes were basically the same. The results would help to clarify the function of <i>BpPIN</i>5 in <i>B. platyphylla</i>.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.01.011