%0 Journal Article
%A Jiao XU
%A Mei-Qi LIU
%A Si-Jia WANG
%A Wei MA
%A Wei SUN
%A Wei-Chao REN
%A Xin-Xin YU
%T Phylogenetic Analysis of DNA Barcoding Sequences in the Genus <i>Salix</i>
%D 2021
%R 10.7525/j.issn.1673-5102.2021.06.007
%J Bulletin of Botanical Research
%P 896-903
%V 41
%N 6
%X <p>We used phylogenetic analysis to compare the ITS2 and psbA-trnH sequences of the plant of the genus <i>Salix</i> samples， so as to explore the genetic diversity and genetic relationship of these resources. Extract genomic DNA of the genus <i>Salix </i>and <i>Tamarix chinensis</i> samples. The ITS2 and psbA-trnH gene sequences of each samples were amplified and sequenced by PCR. The intraspecific and interspecific genetic distances and the neighbor joining（NJ） methods were used to construct phylogenetic trees. According to the phylogenetic tree of ITS2. <i>S. pentandra</i>， <i>S. rorida</i>， <i>S. linearistipularis</i> and <i>S. schwerinii</i> formed a monophyletic clade， distinguished from the same genus. Phylogenetic tree constructed by psbA-trnH sequences were unable to distinguish the genus <i>Salix</i> samples from different geographical sources. Therefore， ITS2 sequence was suitable for the authentication， and would provide a certain reference for the subsequent development and research of the plant of genus <i>Salix.</i></p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.06.007