%0 Journal Article
%A Ke-Xin WU
%A Li-Qiang MU
%A Long-Jie LIU
%A Yun-Fei DIAO
%A Zhong-Yue LI
%T Metabolic Differences Between Wild and Cultivated Species of <i>Taxus cuspidata</i> Based on GC-MS
%D 2021
%R 10.7525/j.issn.1673-5102.2021.05.018
%J Bulletin of Botanical Research
%P 798-806
%V 41
%N 5
%X <p>In order to explore the differences of metabolites between wild and cultivated <i>Taxus cuspidata</i>， nontargeted metabolomics was analyzed. Using GC-MS and multivariate statistical analysis to compare the bark of wild species and cultivated species， the differences in their metabolites were found. A total of 45 significant metabolites were detected， including 4 amino acids， 10 organic acids and some sugar alcohols. Among them， 22 metabolites were up-regulated and the 23 metabolites were down-regulated. KEGG pathway analysis showed that seven pathways had significant effects， including sulfur metabolism， arginine and proline metabolism， pentose phosphate pathway， unsaturated fatty acid biosynthesis， galactose metabolism， alanine， aspartate and glutamic acid metabolism， phenylpropanoid biosynthesis. The metabolites such as amino acids and organic acids were highly expressed in cultivated <i>T. cuspidata</i>. The stress resistance of wild <i>T. cuspidata</i> was weaker than that of cultivated <i>T. cuspidata</i>. However， the expression of carbohydrate was higher in wild species. <i>T. cuspidata</i> wild species had better glucose metabolism ability to resist low temperature stress. The results provided a theoretical basis for the protection of <i>T. cuspidata </i>wild species and stress resistance mechanism of <i>T. cuspidata.</i></p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.05.018