%0 Journal Article
%A Guo-Fan WU
%A Hong-Bin CHENG
%A Juan SHEN
%A Wang-Ze WU
%A Yu-Jun WU
%T Identification of Knockout of <i>BRI</i>1 Mutant in <i>Arabidopsis</i> Mediated by CRISPR/Cas9
%D 2021
%R 10.7525/j.issn.1673-5102.2021.03.006
%J Bulletin of Botanical Research
%P 362-371
%V 41
%N 3
%X <p>CRISPR/Cas9 gene editing technology was used to specificity edit the <i>BRI1</i> of <i>Arabidopsis thaliana</i> to creation new <i>BRI1</i> mutants. We analyzed the sequence of <i>BRI1 </i>in<italic/>the obtained transgenic plants， the results showed that as one new <i>BRI1</i> mutant due to the insertion of new bases， the normal BRI1 protein is terminated prematurely. This mutant have the similar phenotype as <i>bri</i>1-710， and both mutants show impaired response to BL treatment compared with wild-type. Those results suggested that the N-terminal of BRI1 may play a role in the BR signaling pathway. Therefore， this study could provide a reliable reference for further studies on the BRI1 function of <i>A. thaliana</i> and other homologous species.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.03.006