%0 Journal Article
%A Fang-Hui LIU
%A Fu-Zhen ZHANG
%A Jing SUN
%A Rong-Chao GE
%A Xin-Xin CHEN
%T Cloning and Functional Analysis of Rice Gene <i>OsRPK2</i>
%D 2021
%R 10.7525/j.issn.1673-5102.2021.04.016
%J Bulletin of Botanical Research
%P 604-613
%V 41
%N 4
%X <p>Receptor-like protein kinase gene <i>OsRPK1</i> plays an important role in the resistance signal transduction of rice. <i>OsRPK2</i> gene， which is highly homologous to <i>OsRPK1</i>， was amplified and transformed into <i>Arabidopsis</i><i>thaliana</i> by the overexpression vector p1300：35S：<i>OsRPK2</i>. The stress resistance analysis of 35S：<i>OsRPK2</i><i>Arabidopsis</i> homozygous showed that the germination rate of <i>OsRPK2</i> overexpressed <i>Arabidopsis </i>was significantly lower than that of the wild-type under salt， ABA and PEG stress. The root and plant growth of the <i>OsRPK2</i> overexpressed<i> Arabidopsis</i> seedlings showed more obvious inhibition than that of wild-type. Physiological detection of 35S：<i>OsRPK2</i><i>Arabidopsis</i> showed that the chlorophyll content decreased more significantly， proline increased less and malondialdehyde content increased more significantly than the wild-type<italic/>after salt treatment. These physiological mechanisms of <i>OsRPK2</i> overexpression <i>Arabidopsis</i> significantly reduce the stress resistance. Through qRT-PCR detection of the <i>OsRPK2</i> overexpressed <i>Arabidopsis</i>， the overexpression of <i>OsRPK2</i> inhibited the expression of <i>SAD</i>， <i>SOS3</i> and <i>FRY</i> genes significantly. Therefore， <i>OsRPK2</i> gene may affect<i> Arabidopsis</i> resistance through SOS and CDPK signaling pathway.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.04.016