%0 Journal Article
%A De-Li YANG
%A Hai-Long PANG
%A Han-Qing FENG
%A Juan-Juan WANG
%A Kun SUN
%T Effects of Extracellular ATP on the Chitosan-induced Changes in ROS Levels and PAL Activity
%D 2021
%R 10.7525/j.issn.1673-5102.2021.04.014
%J Bulletin of Botanical Research
%P 588-595
%V 41
%N 4
%X <p>In this experiment， tobacco BY-2 suspension cells were used as materials to investigate the effects of extracellular ATP on the chitosan-induced changes of ROS（reactive oxygen species）level and PAL（phenylalanine ammonia-lyase）activity. The results showed that the tobacco suspension cells mixed with chitosan 5-20 μg·mL<sup>-1</sup> increased the intracellular ROS level at adose-dependent manner， and the PAL activity also increased and reached to the maximum at 15 μg·mL<sup>-1</sup> chitosan， and then decreased slightly. There was not significant difference in ROS levels and PAL activity in tobacco suspension cells by adding 10-40 μmol·L<sup>-1</sup> exogenous ATP. The extracellular ATP（eATP） level gradually decreased with the concentration of chitosan increased. The effects of eATP on the chitosan-induced change of ROS level and PAL activity were further analyzed. The results showed that the exogenous ATP 20 μmol·L<sup>-1</sup> added to chitosan-induced tobacco suspension cells effectively alleviated the increases of ROS level and PAL activity in cells. These results showed that effects of extracellular ATP can affect the chitosan-induced changes in ROS level and PAL activity in cells.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.04.014