%0 Journal Article
%A Chao WANG
%A Chuan-Ping YANG
%A Jia-Xin LIU
%A Ying YU
%A Yi-Ping GUO
%T Expression Profile Analysis ofXylem Development Regulated by <i>BpNAC</i>012 Gene from <i>Betula platyphylla</i>
%D 2021
%R 10.7525/j.issn.1673-5102.2021.02.013
%J Bulletin of Botanical Research
%P 251-261
%V 41
%N 2
%X <p>NAC transcription factors have been considered to be the switches that regulate the formation of plant secondary cell walls. Our previous work indicated that the gene<i> BpNAC</i>012 was involved in the secondary cell wall biosynthesis of birch. In order to identify the downstream target genes regulated by <i>BpNAC</i>012， transcriptome sequencing of stems from <i>BpNAC</i>012<italic/>over-expression and RNAi plants was conducted， and the differentially expressed genes were identified compare to the wild type birch. The results showed that， in contrast to the wild type plants， 627 genes were up-regulated and 229 genes were down-regulated in the over-expression transgenic line， and 299 up-regulated genes and 207 down-regulated genes were identified in the RNAi lines. The expression changes of downstream genes in the overexpression transgenic lines were much greater than that in the RNAi plants. Inhibition of <i>BpNAC</i>012 expression significantly affected the expression of genes related to protein modification and transport. The differentially expressed genes were enriched in pathways such as receptor signaling pathways， nutritional metabolism， amino acid synthesis， and phenylpropane biosynthesis. In addition， <i>BpNAC</i>012 can regulate the expression of genes related to cellulose， lignin bio-synthesis and xylem development， as well as a variety of transcription factors. This study laid the foundation for further analysis of the molecular regulatory mechanism of <i>BpNAC</i>012 in secondary cell wall bio-synthesis of birch.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.02.013