An efficient and rapid micro-propagation system using tissue culture for two new varieties “Red Sprite” and “Winter Gold” of Ilex verticillata L. was established with the young shoots as explants， and an optimum method produced large numbers of genetically uniform seedlings and maintained its excellent character for I.verticillata L was offered. The optimal medium for axillary bud induction was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA， and the induction rate of both varieties were 100% respectively. The preferred medium for cluster bud proliferation was MS+0.1 mg·L-1 NAA+0.5 mg·L-1 6-BA+0.5 mg·L-1 CPPU. The highest proliferation coefficient was 5.7 for “Red Sprite”， and 4.5 for “Winter Gold” for 25 d culturing respectively. The optimal seedling medium for “Red Sprite” was MS+0.1 mg·L-1 NAA+0.5 mg·L-1 6-BA+1.0 mg·L-1 TDZ+1.0 mg·L-1 GA， and that of “Winter Gold” was MS+0.1 mg·L-1 NAA+0.5 mg·L-1 6-BA+0.05 mg·L-1 TDZ+1.0 mg·L-1 GA， the seedling height was up to 6.80 and 4.38 cm respectively. The optimal rooting induction medium was 1/2MS+0.4 mg·L-1 NAA+0.4 mg·L-1 IBA+1.0 mg?L-1 activated carbon， the rooting rate of “Red Sprite” and “Winter Gold” were 95.0%， or 97.0% respectively. The best transplanting substrates were peat∶perlite=3∶2， with a survival rate of 95.7% for “Red Sprite” and 96.8% for “Winter Gold” respectively.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.02.010