%0 Journal Article
%A Ying LIU
%T Tissue Culture via Seed Embryo for <i>Michelia macclurei</i>
%D 2021
%R 10.7525/j.issn.1673-5102.2021.01.011
%J Bulletin of Botanical Research
%P 79-88
%V 41
%N 1
%X <p>In order to explore tissue culture and micro-propagation technical system of <i>Michelia macclurei</i>， the seeds from its elite families were used to carry out experimental studies at aspects of explants selection and disinfection， basic medium， PVP concentration， type and concentration of rooting promoter. The results showed that：①The embryos were well sterilized with 0.1% mercury solution for 6-9 min， and the aseptic germination rate could reach 60%-65%； ②LY medium was the best basic medium， and suitable for the proliferation and growth of most embryo lines； ③Application of PVP with concentration of 4-8 g·L<sup>-1</sup> could significantly reduce browning degree of medium and promote growth of the shoots； ④There were significant differences in rooting ability and root