%0 Journal Article
%A Hui-Ga BAO
%A Ren-Hua JI
%A Ri-Ge-Le TE
%A Shu-Lan BAI
%A Wen-Bo ZHANG
%A Xiao-Fei LIN
%A Ying-Liang BAO
%T Subcellular Localization of the Protein Coded by the <i>UDP</i>-Glucose Dehydrogenase Gene from Paper Mulberry and Functional of Its Promoter 5′-end Deletion Fragment
%D 2020
%R 10.7525/j.issn.1673-5102.2020.06.016
%J Bulletin of Botanical Research
%P 932-942
%V 40
%N 6
%X <p>We analyzed the function of <i>cis</i>-acting elements of<i> UDP</i>-Glucose Dehydrogenase Gene from Paper Mulberry（DDBJ， <i>BpUGDH </i>accession No.LC457701）<italic/>promoter， the five different lengths of <i>BpUGDH</i> promoter 5'-terminal deletion fragment were ligated with the <i>GUS</i> gene by using 5'terminal deletion and homologous recombination techniques， and conducted through heterologous expression in <i>Nicotiana benthamiana </i>by agro-infiltration method. In order to locate the specific location of the protein encoded by <i>BpUGDH</i> gene in cells， subcellular localization of BpUGDH protein was carried out by using the fusion target gene of GFP reporter gene. The results show that the sequences within -244 bp can mediate the induction of GUS gene expression， and the region between -973， -465， -355， -281， -244 bp of the <i>BpUGDH</i> promoter were crucial for regulating the promoter activity. Furthermore， BpUGDH was located in chloroplast.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.06.016