%0 Journal Article
%A Bing LI
%A Yu-Xiang CHENG
%T Analysis of <i>SHMT</i> Gene Family and Production of <i>PtrSHMT9</i> Mutant in <i>Populus trichocarpa</i>
%D 2020
%R 10.7525/j.issn.1673-5102.2020.06.013
%J Bulletin of Botanical Research
%P 906-912
%V 40
%N 6
%X <p>Serine hydroxy methyltransferases（SHMTs） played an important role in the one-carbon（C1） metabolism pathway in plant cells. We identified 9<i> SHMT</i> genes from the genome of <i>Populus trichocarpa</i>. Populus eFP resource showed that 7 <i>PtrSHMTs</i> were expressed in multiple tissues， among which <i>PtrSHMT</i>9 was the most highly expressed gene in xylem. By analysis of RT-PCR and Aspwood， <i>PtrSHMT</i>9 had a high level in secondary wall thickening of stem wood tissue， suggested its involvement in poplar wood formation. Using Cas9/gRNA technique， <i>PtrSHMT</i>9-edited mutants were prepared and 4 different <i>ptrshmt</i>9 knockouts were obtained. Our results provided basic information and genetic material for exploring the function of <i>SHMT</i> and <i>PtrSHMT</i>9 in trees.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.06.013