%0 Journal Article
%A Chun-Miao SHAN
%A Jia-Wen WU
%A Ke-Long MA
%A Li-Qiang ZHAO
%A Sheng-Xiang ZHANG
%A Yuan-Yuan SHI
%T Identification of Key Enzyme Genes Involved in Anthocyanin Synthesis pathway in <i>Clinopodium gracile</i> by Transcriptome Analysis
%D 2020
%R 10.7525/j.issn.1673-5102.2020.06.011
%J Bulletin of Botanical Research
%P 886-896
%V 40
%N 6
%X <p>In order to further understand the pathway of anthocyanin synthesis， the four tissues of <i>C.gracile</i>， namely， root， stem， leaf and flower， were sequenced using BGISEQ-500 platform. 128 856 unigenes were assembled and 40 unigenes encoding six key enzymes involved in the anthocyanin biosynthesis were identified. Homologous alignment and structure simulation of bifunctional dihydroflavonol 4-reductase（DFRs） were carried out， which showed that they have well-conserved sequences， spatial structures and NAD+ binding sites. The DFR structure model showed a tetrameric structure with Rossmann-like α-helixes/β-sheets/α-helixes sandwich fold.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.06.011