%0 Journal Article
%A CHANG Cheng-Long
%A LIU Cai-Xia
%A YOU Xiang-Ling
%A ZHANG Xiang
%A ZHENG Lan
%A ZHENG Mi
%A LI Chan
%T Genetic Transformation of <i>PsnHB</i>22 Gene from <i>Populus simonii</i>&#215;<i>P.nigra</i> in Tobacco
%D 2020
%R 10.7525/j.issn.1673-5102.2020.05.015
%J Bulletin of Botanical Research
%P 760-767
%V 40
%N 5
%X HD-Zip gene family plays an important role in light signal transduction, abiotic stress and leaf development. HB22 transcription factor is a member of HD-ZipⅠ subfamily. In order to investigate the function of <i>PsnHB</i>22 gene, <i>PsnHB</i>22 gene was cloned from <i>Populus simonii</i>&#215;<i>P.nigra</i>. The expression vector was transformed into tobacco(<i>Nicotiana tabacum</i>). The phenotype of transgenic tobacco was observed after PCR and qRT-PCR detection. The results showed that the leaves of transgenic tobacco were narrow and the plants were significantly lower than those of wild type tobacco during vegetative growth period. The chlorophyll content of transgenic tobacco and wild-type tobacco leaves was determined. The chlorophyll content of transgenic tobacco was significantly higher than that of wild-type tobacco. <i>PsnHB</i>22 gene plays an important role in plant height growth, photosynthesis and leaf morphogenesis.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.05.015