%0 Journal Article
%A FU Peng-Yue
%A LIU Yi
%A QI Wan-Zhu
%A QU Guan-Zheng
%A ZENG Dong
%A ZHAO Qiu-Shuang
%A ZHAO Xi-Yang
%A WANG Wan-Qi
%T Cloning and Expression Analysis of <i>BpJMJ</i>18 Gene Promoter in <i>Betula platyphylla</i>
%D 2020
%R 10.7525/j.issn.1673-5102.2020.05.014
%J Bulletin of Botanical Research
%P 751-759
%V 40
%N 5
%X In order to investigate the function of <i>BpJMJ</i>18 gene in plant growth and development, the <i>BpJMJ</i>18 promoter gene was cloned from the genome of <i>Betula platyphylla</i> by PCR. By bioinformatics analysis, beside the basic elements such as TATA box and CAAT box, but also light responsive elements and many hormone responsive elements. Plant expression vector pBI101-<i>BpJMJ</i>18pro::<i>GUS</i> was constructed and transient transformed into <i>B.platyphylla</i> by <i>Agrobacterium</i> mediated method; the transgenic lines were analyzed by GUS staining, and the results showed that the promoter of <i>BpJMJ</i>18 gene could drive <i>GUS</i> gene to express in the roots, leaves and stems of <i>B.platyphylla</i>. Therefore, the <i>BpJMJ</i>18 promoter of <i>B.platyphylla</i> has the promoter activity, which may affect the growth and development of plants.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.05.014