%0 Journal Article
%A XIA Ming-Ze
%A ZHANG Fa-Qi
%A ZHANG Yu
%T Transcriptome Analysis for Medicinal Plant <i>Anisodus tanguticus</i>
%D 2020
%R 10.7525/j.issn.1673-5102.2020.03.018
%J Bulletin of Botanical Research
%P 458-467
%V 40
%N 3
%X The transcriptome sequencing analysis of <i>Anisodus tanguticus</i> was carried out by high throughput sequencing technology to understand resource plant <i>A.tanguticus</i> deeply. The 71 463 Unigenes were obtained after processing. By comparing with several databases,we classified and analyzed the genes, and finally succeeded in annotating 47 624 genes. The 13 110 genes with 26 subclasses were annotated after comparing with KOG protein library.Compared with NR library, 39 621unigenes were annotated.The transcripts were compared with Swissprot and TrEMBL to obtain GO functional annotations. The 29 309 unigenes obtained from the annotations could be divided into three categories:molecular function, biological process, and cellular components, with 62 subcategories.Referring to the KEGG database, 3 679 genes were annotated. The metabolic pathways involved can be classified into four categories:metabolic related pathways, genetic information processing,cellular processes, and environmental information processing. Among them,metabolic related pathways are the most, accounting for about half of all metabolic pathways.Statistical results of metabolic pathways and related unigenes of the active ingredients of <i>A.tanguticus</i> showed that the number of metabolic pathways related to alkaloids was the most, and the number of unigenes corresponding to terpenoids and phenylpropanoids was the most.In addition, 31 382 SNP loci and 6 SSR repeat types were detected. Among them, single base repeat types accounted for the highest proportion, with 56.52 SSR repeats per million bases, accounting for 45.30%.These results enrich the transcriptome information of <i>A.tanguticus</i> and lay a foundation for the study of the molecular biology, which contribute to the further development and utilization of <i>A.tanguticus</i>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.03.018