%0 Journal Article
%A CAO Sen-Lin
%A FAN Zuo-Yi
%A KONG Ling-Yuan
%A LI Xi-Peng
%A MENG Qing-Gang
%A WANG De-Qiu
%A WANG Jing-Yuan
%A WANG Qi
%A ZHAO Xi-Yang
%A LI Xiang
%T Codon Usage Bias of Chalcone Synthase Gene <i>CHS</i> in <i>Pinus koraiensis</i>
%D 2020
%R 10.7525/j.issn.1673-5102.2020.03.017
%J Bulletin of Botanical Research
%P 447-457
%V 40
%N 3
%X Chalcone synthase(<i>CHS</i>) is widely found in plants and is a key enzyme in anthocyanin synthesis pathway. It can catalyze the binding of malonyl coenzyme A and coumaryl coenzyme A to generate flavonoids. CodonW and EMBOSS were used to analyze the codon usage bias of <i>CHS</i> gene in <i>Pinus koraiensis</i>. The <i>CHS</i> gene of <i>P.koraiensis</i> were compared with those of the other 24 plants and model plant genome, which laid a foundation for the selection of appropriate expression system. The effective codon number(ENC) and GC content of <i>CHS</i> gene coding region were 48.92 and 0.351, respectively. The content of C+G was higher than that of A+T, and the codon preferred to end with A/T. The G+C content of most plants <i>CHS</i> genes is higher than that of A+T, and the codon preferred to end with C/G. By cluster analysis, the codon usage bias of <i>P.koraiensis</i> was similar to <i>P.massoniana</i> and <i>P.densiflora</i> pdchsx. In addition, it was found that <i>Arabidopsis thaliana</i> and <i>Escherichia coli</i> might be ideal receptor systems for <i>CHS</i> genetic transformation and heterogeneous expression.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.03.017