%0 Journal Article
%A CEN Xi
%A LIN Jian-Hui
%A LIU Sheng-Yi
%A LIU Zi-Guang
%A SUN Shi-Chen
%A WU Juan
%A ZHANG Nan
%A YANG He
%T Expression and Function of <i>BoNR</i>8 lncRNA in Cabbage under Cold Stress
%D 2020
%R 10.7525/j.issn.1673-5102.2020.03.016
%J Bulletin of Botanical Research
%P 441-446
%V 40
%N 3
%X Long non-coding RNA(lncRNA) with longer than 200 bp in length do not contain open reading frames, and they generally do not have a protein-coding function. They are ubiquitous in the body and play a variety of biological roles. There are few studies on lncRNA specifically expressed in the germination stage of plant seeds. <i>BoNR</i>8 lncRNA is a long non-coding RNA(about 272 bp) transcribed by RNA polymerase Ⅲ in cabbage. Previous studies have found that it is specifically expressed in the seed germination stage, and the overexpression of <i>BoNR</i>8 lncRNA in Arabidopsis inhibits seed germination under normal conditions and reduces the sensitivity of germinated seeds to ABA. In this study, we analyzed the sequence of <i>BoNR</i>8 lncRNA and found that there was a cold stress motif(ATATAAATAAAT) in the transcribed region, and this motif exists in the largest stem loop of the secondary structure of <i>BoNR</i>8 lncRNA. Biochemical experiments further showed that <i>BoNR</i>8 lncRNA responded to low temperature environment, and its overexpression inhibited seed germination of Arabidopsis thaliana under low temperature stress. The discovery of low-temperature related functions of <i>BoNR</i>8 lncRNA provides new materials for low temperature resistance research of plant and enriches the study of low temperature resistance mechanism of plant.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.03.016