%0 Journal Article
%A GUAN Qing-Jie
%A HE Ming-Liang
%A LI Xiu-Feng
%A LIAO Xu
%A LIU Chen-Xi
%A LIU Jia-Li
%T Zinc Finger Protein <i>OsZFP</i>6 Expression Features and Functions in Saline-alkali Stress Response
%D 2020
%R 10.7525/j.issn.1673-5102.2020.03.014
%J Bulletin of Botanical Research
%P 424-432
%V 40
%N 3
%X C2HC type zinc finger protein was screened, encoded 304 amino acids and named OsZFP6. The expression level of <i>OsZFP</i>6 was increased by abiotic stress, including alkali(NaHCO<sub>3</sub>) and H<sub>2</sub>O<sub>2</sub> treatment in two-week old rice leaves but decreased in roots. Thus, it was speculated that OsZFP6 was involved in the regulation corresponding reaction pathways of resistance. We fused a green fluorescent protein(GFP) with it and found that OsZFP6 was located in the nucleus. Moreover, rice overexpressed <i>OsZFP</i>6 were more tolerant to NaHCO<sub>3</sub> treatments. The overexpressed plants had strong tolerance, and were higher than WT in plant height and fresh weight, but the contents of MDA and H<sub>2</sub>O<sub>2</sub> in vivo were lower than non-transgenic plants, which indicated that OsZFP6 could enhance the resistance of plants by scavenging peroxide in response to NaHCO<sub>3</sub> stress. Preliminarily, it was speculated that <i>OsZFP</i>6 gene participated in the regulation of resistance and it may play a vital role in transcription regulation.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.03.014