%0 Journal Article
%A CHEN Zhe
%A LI Yong-Xia
%A SHEN Xi-Long
%A WANG Yao
%A WU Wei-Feng
%A YANG Ding-Yuan
%A ZHANG Qun-Ying
%T Preliminary Selection of Potential DNA Barcodes for <i>Rubus</i>
%D 2020
%R 10.7525/j.issn.1673-5102.2020.02.018
%J Bulletin of Botanical Research
%P 301-307
%V 40
%N 2
%X In order to establish a molecular identification technique of DNA barcoding for <i>Rubus</i>, and screening universal barcode sequences suitable for <i>Rubus</i>. With GenBank data we analyzed the genetic variation, barcoding gap and NJ consensus tree of six DNA barcoding sequences that contain ITS, ITS2, <i>matK, rbcL, trnH-psbA</i> and <i>trnL-trnF</i>. The intraspecific variation and interspecies variation of <i>trnH-psbA, matK, rbcL</i> and <i>rtnL-trnF</i> were different, and the variation resolutions were 97.32%, 83.33%, 79.07% and 64.95%, respectively. And <i>trnH-psbA, matK, rbcL</i> and <i>rtnL-trnF</i> had obvious barcoding gap; NJ consistent tree showed that <i>matK</i> had the highest proportion of solopathogenicity(67%), and then <i>trnH-psbA</i>(64%), <i>rtnL-trnF</i>(43%), <i>rbcL</i>(30%). The intraspecific variation and interspecies of <i>matK</i> and <i>trnH-psbA</i> sequences for <i>Rubus</i> are different, which can distinguish different species well with great identification potential. It is recommended to use <i>matK</i> and <i>trnH-psbA</i> as the core barcode sequences identified for <i>Rubus</i>, and <i>rtnL-trnF</i> and <i>rbcL</i> are used as auxiliary barcode sequences.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.02.018