%0 Journal Article
%A CHENG Yu-Xiang
%A WANG Shuang
%A XIA De-An
%T Identification of Key Amino Acid Sites in <i>AtGDPD-Like</i>3 with Role in <i>Arabidopsis</i> Root Hairs
%D 2020
%R 10.7525/j.issn.1673-5102.2020.01.012
%J Bulletin of Botanical Research
%P 79-84
%V 40
%N 1
%X <i>AtGDPD-Like</i>3 encode glycerophosphodiester phosphodiesterase-like(GDPD) proteins and shv3, its family gene <i>AtGDPD-Like</i>3 mutant, has serious root hair defects. To identify key amino acid sites in AtGDPD-Like3, we constructed S<sup>538</sup>A, V<sup>556</sup>A and D<sup>628</sup>A single site mutation of <i>AtGDPD-Like</i>3, which were expressed in <i>atgdpdl</i>3 mutants, respectively. The results showed that <i>AtGDPD-Like</i>3 with V<sup>556</sup>A or D<sup>628</sup>A mutation could completely restore root hairs growth defects of <i>atgdpdl</i>3, whereas, <i>AtGDPD-Like</i>3 with S<sup>538</sup>A mutation partially restored root hairs growth defects. These indicate that Ser<sup>538</sup> is the key amino acid site in AtGDPD-Like3, which affects function of its protein. Meanwhile, it implies other key amino acid sites in AtGDPD-Like3. The results of this study would lay a foundation for further exploring the functional mechanism of AtGDPD-Like3 protein.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.01.012